Experientia

, Volume 43, Issue 6, pp 601–602 | Cite as

Phospholipase C activation via a GTP-binding protein in tumoral islet cells stimulated by carbamylcholine

  • F. Blachier
  • W. J. Malaisse
Short Communications

Summary

Carbamylcholine and GTP act synergistically in stimulating the production of [3H]inositol-1-phosphate by digitonized tumoral islet cells (RINm5F line) prelabeled with myo-[2-3H(N)]inositol. The response to these two agents is similar to that evoked by GTPγS. These findings suggest that a GTP-binding regulatory protein couples the occupancy of muscarinic receptors to activation of phospholipase C in pancreatic islet cells.

Key words

Phospholipase C GTP-binding protein carbamylcholine 

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Copyright information

© Birkhäuser Verlag Basel 1987

Authors and Affiliations

  • F. Blachier
    • 1
  • W. J. Malaisse
    • 1
  1. 1.Laboratory of Experimental MedicineBrussels Free UniversityBrussels(Belgium)

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