Summary
A gelatin sponge model system for tumor cell inoculation and retrieval of tumor-associated leukocytes is described. Gelatin sponges pre-implanted in nude mice harboring tumorigenic Chinese hamster ovary cells (line CHO) were examined at 2 and 11 days after injection of tumor cells for tumor cell content and leukocyte accumulation after digesting the sponge matrix in collagenase solution.
The data indicate a progressive influx of host cells consisting primarily of macrophages, neutrophils and lymphocytes. The total number of viable tumor cells as well as the fraction of surviving tumor cells with clonogenic potential also increased with tumor age. Blank sponges not harboring tumor cells elicited an inflammatory response in the animals which did not change appreciably with length of sponge residence. However, when the sponges were harboring tumor cells, the accumulation of host leukocytes far exceeded that which occurred in blank sponges. This observation suggests a host response directed toward the tumor which is absent in animals bearing blank sponges. Apart from providing anchorage for injected cells, the gelatin sponge, by virtue of its digestibility in collagenase, makes possible the easy retrieval and precise quantitation of tumor-associated host cells.
Similar content being viewed by others
Literatur
Evans, R., Cancer Met. Rev.1 (1982) 227.
Kreider, J.W., Bartlett, G.L., and Butkiewicz, B.L., Cancer Met. Rev.3 (1984) 53.
Roberts, P.J., and Hayry, P., Transplantation21 (1976) 437.
Wiktorowicz, K., Roberts, P.J., and Hayry, P., Cell. Immun.38 (1978) 255.
Vallera, D.A., Mentzer, S.J., and Maizel, S.E., Cancer Res.42 (1982) 397.
Wells, R.S., Campbell, E.W., Swartzendruber, D.E., Holland, L.M., and Kraemer, P.M., J. natn. Cancer Inst.69 (1982) 415.
Kraemer, P.M., Travis, G.L., Ray, F.A., and Cram, L.S., Cancer Res.43 (1983) 4822.
Kraemer, P.M., Travis, G.L., Saunders, G.C., Ray, F.A., Stevenson, A.P., Bame, K., and Cram, L.S., Proceedings of the 4th International workshop on immune-deficient animals, p. 214. Ed. B. Sordat. S. Karger, Basel 1984.
Ascher, N.L., Hoffman, R., Chen, S., and Simmons, R.L., Cell. Immun.52 (1980) 38.
Cram, L.S., Bartholdi, M.F., Ray, F.A., Travis, G.L., and Kraemer, P.M., Cancer Res.43 (1983) 4828.
Kerkof, P.K., J. Tissue Cult. Meth.7 (1982) 23.
Akporiaye, E.T., Stewart, S., and Stewart, C.C., J. immun. Meth.75 (1984) 149.
Steinkamp, J.A., Orlicky, D.A., and Crissman, H.A., J. Histochem. Cytochem.27 (1979) 273.
Hiebert, R.D., Jett, J.H., and Salzman, G.C., Cytometry1 (1981) 337.
Stewart, C.C., Lin, H.S., and Adles, C. J. exp. Med.141 (1975). 1114.
Walker, E.B., Akporiaye, E.T., Warner, N.L., and Stewart, C.C., J. Leukocyte Biol.37 (1985) 121.
Mulcahy, R.T., Rose, D.P., Mitchen, J.M., and Clifton, K.H., Endocrinology106 (1980) 1769.
Gould, M.N., Biel, W.F., and Clifton, K.H., Exp. Cell Res.107 (1977) 405.
Russel, S.H., Doe, W.F., Hoskins, R.G., and Cochrane, C.G., Int. J. Cancer18 (1976) 322.
Author information
Authors and Affiliations
Additional information
Supported by the United States Department of Energy and National Institutes of Health Grant P41-RR01315.
Rights and permissions
About this article
Cite this article
Akporiaye, E.T., Saunders, G.C. & Kraemer, P.M. A gelatin sponge model for studying tumor growth: quantitation of tumor cells and leukocytes in the CHO tumor. Experientia 43, 589–593 (1987). https://doi.org/10.1007/BF02126340
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02126340