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Juvenile hormone catabolism inManduca sexta: Homologue selectivity of catabolism and identification of a diol-phosphate conjugate as a major end product

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Abstract

We studied time-dependent metabolism of (10R)-[3H] juvenile hormone (JH) III and (10R, 11S)-[3H]JH I injected intoManduca sexta larvae; the hormones are metabolized to polar metabolites, expecially the JH acid-diol, and an unknown. Products were analyzed using a reversed-phase liquid chromatography assay. (10R)-JH III is metabolized much more rapidly than (10R, 11S)-[3H]JH I, whether injected seperately or as a mixture of hormones. The unknown metabolites of JH I and JH III were identified as phosphate conjugates of JH I and JH III diol by tandem mass spectral analysis of isolated samples. The phosphate conjugate of JH I diol is the principle end product of JH I metabolism.

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Authors and Affiliations

  1. Department of Biochemistry, University of Nevada, 89557, Reno, Nevada, USA

    P. P. Halarnkar, G. P. Jackson & D. A. Schooley

  2. Syntex Research, 94304, Palo Alto, California, USA

    K. M. Straub

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  1. P. P. Halarnkar
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  2. G. P. Jackson
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  3. K. M. Straub
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  4. D. A. Schooley
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Halarnkar, P.P., Jackson, G.P., Straub, K.M. et al. Juvenile hormone catabolism inManduca sexta: Homologue selectivity of catabolism and identification of a diol-phosphate conjugate as a major end product. Experientia 49, 988–994 (1993). https://doi.org/10.1007/BF02125646

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