Abstract
Non volatile N, N-dimethylformamide (DMF) at room temperature is proposed as a new solvent for determining the chlorophylla in cultured and natural phytoplankton samples. Advantages of DMF compared to the solvents ordinarily used are (1) higher extraction efficiency, (2) rapid extraction time of 30 min, (3) simple extraction procedure requiring neither extra heating nor grinding, but soaking, and (4) a long stable life of chlorophylla (at least one month at 5°C in the dark).
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Suzuki, R., Ishimaru, T. An improved method for the determination of phytoplankton chlorophyll using N, N-dimethylformamide. Journal of the Oceanographical Society of Japan 46, 190–194 (1990). https://doi.org/10.1007/BF02125580
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DOI: https://doi.org/10.1007/BF02125580