Medical Microbiology and Immunology

, Volume 169, Issue 4, pp 281–289 | Cite as

Interferon induces an antiviral state in ganglioside-deficient transformed mouse fibroblasts

  • D. Schiffmann
  • K. Koschel


The antiviral activity of mouse fibroblast interferon against vesicular stomatitis virus was investigated in L-929 mouse fibroblasts and the ganglioside-deficient L-929 mutant cells (ATCC clone NCTC 2071). Although it has been widely reported that gangliosides serve as primary receptors for interferon at the cellular membrane, only a small difference in interferon sensitivity was observed between the wild-type L-929 and the ganglioside-deficient NCTC 2071 cells. It was not possible, however, to overcome this difference by administration of exogenous gangliosides.


Interferon Small Difference Antiviral Activity Cellular Membrane Mutant Cell 
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  1. Aguet M (1980) High affinity binding of 125J-labelled mouse interferon to a specific cell surface receptor. Nature 284:459–461PubMedGoogle Scholar
  2. Ankel H, Chany C, Galliot B, Chevalier MJ, Robert M 1973, Antiviral effect of interferon covalently bound to sepharose. Proc Natl Acad Sci US 70:2360–2363Google Scholar
  3. Ankel H, Krishnamurti C, Besancon F, Stefanos S, Falcoff E (1980) Mouse fibroblast (type I) and immune (type II) interferons: Pronounced differences in affinity for ganliosides and in antiviral and antigrowth effects on Mouse Leukemia L 1210 R cells, Proc Natl Acad Sci Us 77:2528–2532Google Scholar
  4. Belardelli F (1980) Cholera toxin and its B subunit inhibit interferon effects on Friend cells. Abstracts of the 13th FEBS Meeting Jerusalem 167Google Scholar
  5. Besancon F, Ankel H (1974a) Inhibition of interferon action by plant lectins. Nature 250:784–786PubMedGoogle Scholar
  6. Besancon F, Ankel H (1974b) Binding of interferon to gangliosides. Nature 252:478–480PubMedGoogle Scholar
  7. Besancon F, Ankel H (1976) Specificity and reversibility of interferon ganglioside interaction. Nature 259:576–578PubMedGoogle Scholar
  8. Besancon F, Ankel H (1977) Membrane receptors for interferon. Texas Reports on Biology and Medicine 35:282–292PubMedGoogle Scholar
  9. Chang EH, Jay TF, Friedman RM (1978) Physical, morphological and biochemical alterations in the membranes of AKR mouse cells after interferon treatment. Proc Natl Acad Sci US 75:1859–1863Google Scholar
  10. Chany C (1976) Membrane bound interferon specific cell receptor system: Role in the establishment and amplification of the antiviral state. Biomedicine 24:148–157PubMedGoogle Scholar
  11. Degre M, Hovig T (1976) Functional and ultrastructural studies of the effects of human interferon and cell membranes of in vitro cultured cells. Acta Pathol Microbiol Scand Section B 84:347–358Google Scholar
  12. Degre M (1980) Adsorption of interferon to homologous and heterologous cells. Arch Virol 65:71–76PubMedGoogle Scholar
  13. Friedman RM, Kohn LD (1976) Cholera toxin inhibits interferon action. Biochem Biophys Res Commun 70:1078–1084PubMedGoogle Scholar
  14. Friedman RM, Grollman EF, Chang EH, Kühn LD, Lee G, Jav FT (1977) Interferon and glycoprotein hormones. Texas Reports on Biology and Medicine 35:326–335PubMedGoogle Scholar
  15. Friedman RM (1978) Interferon action and the cell surface. Pharm Therap Acta 2:425–438Google Scholar
  16. Gahmberg CG, Hakomori SI (1973) External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem 248:4311–4317PubMedGoogle Scholar
  17. Gilman AG (1970) A protein binding assay for adenosine 3′:5′-cyclic momophosphate. Proc Natl Acad Sci US 67:305–312Google Scholar
  18. Grollman EF, Lee G, Ramos S, Lazo PS, Kaback HR, Friedman RM, Kohn LD (1978) Relationships of the structure and function of the interferon receptor to hormone receptors and establishment of the antiviral state. Cancer Res 38:4172–4185PubMedGoogle Scholar
  19. Hovanessian AG, Meyrs E, Aujean O, Vaquero C, Stefanos S, Falcoff E (1980) Antiviral response and induction of specific proteins in cells treated with immune T (Type II) interferon analogous to that from viral interferon (Type I)-treated cells. Virology 104:195–204PubMedGoogle Scholar
  20. Knight EJ, Korant BD (1977) A cell surface alteration in Mouse L-cells induced by interferon. Biochem Biophys Res Commun 74:707–713PubMedGoogle Scholar
  21. Knight E (1978) Purification of interferons. Pharm Therap Acta 2:439–446Google Scholar
  22. Kohn LD, Friedman RM, Holmes JM, Lee G (1976) Use of thyrotropin and cholera toxin to probe the mechanism by which interferon initiates its antiviral activity. Proc Natl Acad Sci US 73:3695–3699Google Scholar
  23. Kohno S, UminoY, Saito S (1977) Specificity of binding of interferon to cells. Texas Reports on Biology and Medicine 35:293–315PubMedGoogle Scholar
  24. Kuwata F, Handa S, Fuse A, Morinaga N (1978) Effect of glycolipids detectable in transformed human cells on interferon activities. Biochem Biophys Res Commun 85:77–84PubMedGoogle Scholar
  25. Moss J, Fishman PH, Manganiello VC, Vaughan M, Brady RO (1976) Functional incorporation of ganglioside into intact cells: Induction of choleragen responsiveness. Proc Natl Acad Sci US 73:1034–1037Google Scholar
  26. Pitha PM, Vengris VE, Reynolds FH (1976) The role of cell membrane in the antiviral effect of interferon. J Supramol Struct 4:467–473PubMedGoogle Scholar
  27. Revel M, Bash D, Ruddle FH (1976) Antibodies to a cell surface component coded by human chromosome 21 inhibit action of interferon. Nature 260:139–141PubMedGoogle Scholar
  28. Taki T, Hirabayashi Y, Matsumoto M, Kiyohide K (1979) Enzymic synthesis of a new type of fucose-containing glycolipid with fucosyl-transferase of Rat Ascites Hepatoma cell, AH 7974 F.Biochim Biophys Acta 572:105–112Google Scholar
  29. Tan YH, Schneider EL, Tischfield J, Epstein CJ, Ruddle FH (1974) Human chromosome 21 dosage:Effect on the expression of the interferon induced antiviral state. Science 186:61–63PubMedGoogle Scholar
  30. Vengris VE, Reynolds FH, Hollenberg MD, Pitha PM (1976) Interferon action: Role of membrane gangliosides. Virology 72:486–493PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • D. Schiffmann
    • 1
  • K. Koschel
    • 1
  1. 1.Institut für Virologie und Immunobiologie der Universität WürzburgWürzburgFederal Republic of Germany

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