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An adoptive transfer system for the investigation of granuloma formation in murine listeriosis

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Abstract

A system is described for studying adoptive transfer of granuloma formation in infection of mice with the facultative intracellular bacterium,Listeria monocytogenes. Intravenous injection of graded numbers ofL. monocytogenes-immune peritoneal exudate T-enriched cells (PETLEs) together with 5×104 livingL. monocytogenes resulted in dose-dependent accelerated granuloma formation in the livers of recipient mice. The lymphoid cells conferring granuloma formation were T cells by virtue of their nonadherence to nylon wool and sensitivity to anti-Thy 1.2 antiserum plus complement. Since granuloma formation could not be transferred from C57Bl/6J donors to BALB/c recepients it is concluded that adoptive transfer of granuloma formation is genetically restricted.

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Authors and Affiliations

  1. Institut für Medizinische Mikrobiologie, Freie Universität Berlin, Hindenburgdamm 27, D-1000, Berlin 4

    H. Näher, U. Sperling, H. Skupin-Schüssler & H. Hahn

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  1. H. Näher
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  2. U. Sperling
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  3. H. Skupin-Schüssler
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  4. H. Hahn
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Supported in part by DFG grant Na 163/1-1 to H. Näher and U. Sperling

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Näher, H., Sperling, U., Skupin-Schüssler, H. et al. An adoptive transfer system for the investigation of granuloma formation in murine listeriosis. Med Microbiol Immunol 173, 311–318 (1985). https://doi.org/10.1007/BF02125035

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  • DOI: https://doi.org/10.1007/BF02125035

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