Medical Microbiology and Immunology

, Volume 172, Issue 2, pp 87–99 | Cite as

Establishment of spontaneously outgrowing lymphoblastoid cell lines with Cyclosporin A

  • M. v. Knebel Doeberitz
  • G. W. Bornkamm
  • H. zur Hausen
Article

Abstract

To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cells, either at the onset of cultivation or after 3 weeks. The highest frequency of spontaneous outgrowth of EBV-transformed cells was obtained in unseparated cultures when the addition of CSA was delayed. The presence of unimpaired T cells at the onset of the culture period appeared to have a favourable effect on the spontaneous outgrowth of EBV-carrying lymphoblastoid cell lines.

Keywords

Blood Cell Culture Condition Cyclosporin Culture Period Favourable Effect 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • M. v. Knebel Doeberitz
    • 1
  • G. W. Bornkamm
    • 1
  • H. zur Hausen
    • 1
  1. 1.Institut für VirologieZentrum für Hygiene der Universität FreiburgFreiburgFederal Republic of Germany

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