Establishment of spontaneously outgrowing lymphoblastoid cell lines with Cyclosporin A
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To establish spontaneously outgrowing EBV (Epstein-Barr virus) transformed lymphoid cell lines from healthy EBV-seropositive adults different culture conditions were investigated. The effect of T-cell depletion was compared with that of adding Cyclosporin A (CSA) to unseparated mononuclear blood cells, either at the onset of cultivation or after 3 weeks. The highest frequency of spontaneous outgrowth of EBV-transformed cells was obtained in unseparated cultures when the addition of CSA was delayed. The presence of unimpaired T cells at the onset of the culture period appeared to have a favourable effect on the spontaneous outgrowth of EBV-carrying lymphoblastoid cell lines.
KeywordsBlood Cell Culture Condition Cyclosporin Culture Period Favourable Effect
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- Berger R, Meingassner JG, Knapp W (1983) In vitro effects of Cyclosporin A on human B cell responses. Scand J Immunol (in press)Google Scholar
- Hess AD, Tutschka J, Santos GW (1982) Effect of Cyclosporin A on human lymphocyte responses in vitro. III. CSA inhibits the production of T lymphocyte growth factors in secondary mixed lymphocyte responses but does not inhibit the response of primed lymphocytes to TCGF. J Immunol 128:355–359PubMedGoogle Scholar