The metabolic fate of the 17β-ketol side chain of (21-3H) prednisolone was studied with an enzyme preparation from male golden hamster liver. The acidic metabolite of prednisolone was identified by mass spectrometry as 11β, 17α,20ξ-trihydroxy-3-oxo-1,4-pregnadien-21-oic acid. The enzyme showed substrate specificity, depending on the nature of substituent on the steroid nucleus.
Enzyme Mass Spectrometry Steroid Prednisolone Substrate Specificity
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