Experientia

, Volume 33, Issue 2, pp 253–254 | Cite as

Acidic metabolite of prednisolone

  • H. J. Lee
Article

Summary

The metabolic fate of the 17β-ketol side chain of (21-3H) prednisolone was studied with an enzyme preparation from male golden hamster liver. The acidic metabolite of prednisolone was identified by mass spectrometry as 11β, 17α,20ξ-trihydroxy-3-oxo-1,4-pregnadien-21-oic acid. The enzyme showed substrate specificity, depending on the nature of substituent on the steroid nucleus.

Keywords

Enzyme Mass Spectrometry Steroid Prednisolone Substrate Specificity 

References

  1. 2.
    C. M. Southcott, S. K. Gondoss, A. D. Barker, H. E. McIntosh and M. Darrach, Can. J. Biochem. Physiol.34, 146 (1956).PubMedGoogle Scholar
  2. 3.
    C. H. Gray and D. H. Shaw, J. Endocr.33, 33 (1965).PubMedGoogle Scholar
  3. 4.
    H. Bradlow, B. Zumoff, C. Monder, H. J. Lee and L. Hellman, J. clin. Endocr. Metab.37, 811 (1973).PubMedGoogle Scholar
  4. 5.
    A. C. Day and I. R. Senciall, J. Steroid Biochem.7, 167 (1976).PubMedGoogle Scholar
  5. 6.
    H. J. Lee, K. O. Martin and C. Monder, Fed. Proc. USA34, 2516 (1975).Google Scholar
  6. 7.
    P. K. Willingham and C. Monder, Steroids22, 539 (1973).PubMedGoogle Scholar
  7. 8.
    H. J. Lee, R. Roboz and C. Monder, 23rd Conf. Mass Spec. PSA5 (1976).Google Scholar

Copyright information

© Birkhäuser Verlag 1977

Authors and Affiliations

  • H. J. Lee
    • 1
  1. 1.School of PharmacyFlorida Agricultural and Mechanical UniversityTallahasseeUSA

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