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Cyclic nucleotide levels in the perfused rat heart subjected to hypoxia

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Summary

Isolated rat hearts were subjected to hypoxic perfusion on a recirculating Langendorff apparatus. Following a 30-min-period of aerobic stabilization the hearts were perfused for 30 min with media equilibrated with 84% N2, 12% O2 and 4% CO2. At the end of the hypoxic period myocardial concentrations of cyclic AMP and cyclic GMP were determined by radioimmunoassay. Exposure to hypoxia resulted in a significant increase in cyclic AMP (p<0.01) and a decrease in cyclic GMP (p<0.05) as compared to hearts perfused for 60 min with media gassed with 96% O2, 4% CO2.

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Author notes

  1. R. Nesher

    Present address: Division of Metabolism, Washington University School of Medicine. Barnes: Wohl Hospitals, 63110, St. Louis, Missouri, USA

Authors and Affiliations

  1. Department of Physiological Chemistry, Veterinary Pathology and Pathology, Ohio State University, 43210, Columbus, Ohio, USA

    R. Nesher, W. F. Robinson, L. Gibb, S. P. Bishop & F. A. Kruger

Authors
  1. R. Nesher
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  2. W. F. Robinson
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  3. L. Gibb
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  4. S. P. Bishop
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  5. F. A. Kruger
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Additional information

Acknowledgment. We gratefully acknowledge the excellent technical assistance provided by Mrs Inger O. Boggs and Miss Patricia C. Hannigan. This work was supported in part by U. S. Public Health Service grant HL 14661 from the National Heart and Lung Institute, and by a grant from the Central Ohio Heart Chapter.

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Nesher, R., Robinson, W.F., Gibb, L. et al. Cyclic nucleotide levels in the perfused rat heart subjected to hypoxia. Experientia 33, 215–217 (1977). https://doi.org/10.1007/BF02124074

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