, Volume 33, Issue 2, pp 182–184 | Cite as

Isolation and fusion studies on protoplasts from pollen tetrads

  • P. C. Deka
  • A. K. Mehra
  • N. N. Pathak
  • S. K. Sen


different enzymes were tested for isolation of intact protoplasts from pollen tetrads. About 80% isolation was achieved from pollen tetrads of Cajanus cajan and Zea mays and about 60% from Luffa cylindrica and Lycopersicon esculentum after 4 h of treatment with 5% cellulase. When these mononucleate protoplasts were incubated in presence of 0.05 M CaCl2 in 0.3 M glucose at pH 10.5, 70–80% fusion was achieved. Fusion was rare in sodium nitrate solutions.


Glucose Enzyme Sodium Nitrate CaCl2 
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Copyright information

© Birkhäuser Verlag 1977

Authors and Affiliations

  • P. C. Deka
    • 1
  • A. K. Mehra
    • 1
  • N. N. Pathak
    • 1
  • S. K. Sen
    • 1
  1. 1.Botany DepartmentBose InstituteCalcutta(India)

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