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Experientia

, Volume 33, Issue 2, pp 182–184 | Cite as

Isolation and fusion studies on protoplasts from pollen tetrads

  • P. C. Deka
  • A. K. Mehra
  • N. N. Pathak
  • S. K. Sen
Article

Summary

different enzymes were tested for isolation of intact protoplasts from pollen tetrads. About 80% isolation was achieved from pollen tetrads of Cajanus cajan and Zea mays and about 60% from Luffa cylindrica and Lycopersicon esculentum after 4 h of treatment with 5% cellulase. When these mononucleate protoplasts were incubated in presence of 0.05 M CaCl2 in 0.3 M glucose at pH 10.5, 70–80% fusion was achieved. Fusion was rare in sodium nitrate solutions.

Keywords

Glucose Enzyme Sodium Nitrate CaCl2 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Birkhäuser Verlag 1977

Authors and Affiliations

  • P. C. Deka
    • 1
  • A. K. Mehra
    • 1
  • N. N. Pathak
    • 1
  • S. K. Sen
    • 1
  1. 1.Botany DepartmentBose InstituteCalcutta(India)

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