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Medical Microbiology and Immunology

, Volume 170, Issue 1, pp 37–43 | Cite as

Stabilization of adenovirus infectivity against thermoinactivation by lowered ionic strength

  • Gabriele Sprunck
  • R. Wigand
Article

Abstract

The thermostability of adenovirus 5 increases when the ionic strength is decreased before heating by dilution in distilled water (1∶100, heating at 50‡C). A predilution in Eagle's MEM before heating or a previous dialysis against water had no effect on thermostability. The stabilizing effect was found like-wise in crude and purified virus, in heating at 50‡C up to 320 min, from 50‡C to 56‡C, and in dilution from 10−2 to 10−5. The degree of stabilization was similar to cationic stabilization in 2M NaCl. The infectivity of heated adenovirus 5 was not sensitive to DNase digestion. Other adenovirus types were stabilized in a similar manner.

Keywords

Ionic Strength Lowered Ionic Strength Adenovirus Infectivity Adenovirus Type DNase Digestion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Gabriele Sprunck
    • 1
  • R. Wigand
    • 1
  1. 1.Institut für Hygiene und Mikrobiologie, Abteilung für VirologieUniversitÄtsklinikenHomburg (Saar)Federal Republic of Germany

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