The thermostability of adenovirus 5 increases when the ionic strength is decreased before heating by dilution in distilled water (1∶100, heating at 50‡C). A predilution in Eagle's MEM before heating or a previous dialysis against water had no effect on thermostability. The stabilizing effect was found like-wise in crude and purified virus, in heating at 50‡C up to 320 min, from 50‡C to 56‡C, and in dilution from 10−2 to 10−5. The degree of stabilization was similar to cationic stabilization in 2M NaCl. The infectivity of heated adenovirus 5 was not sensitive to DNase digestion. Other adenovirus types were stabilized in a similar manner.
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