Medical Microbiology and Immunology

, Volume 170, Issue 1, pp 37–43 | Cite as

Stabilization of adenovirus infectivity against thermoinactivation by lowered ionic strength

  • Gabriele Sprunck
  • R. Wigand


The thermostability of adenovirus 5 increases when the ionic strength is decreased before heating by dilution in distilled water (1∶100, heating at 50‡C). A predilution in Eagle's MEM before heating or a previous dialysis against water had no effect on thermostability. The stabilizing effect was found like-wise in crude and purified virus, in heating at 50‡C up to 320 min, from 50‡C to 56‡C, and in dilution from 10−2 to 10−5. The degree of stabilization was similar to cationic stabilization in 2M NaCl. The infectivity of heated adenovirus 5 was not sensitive to DNase digestion. Other adenovirus types were stabilized in a similar manner.


Ionic Strength Lowered Ionic Strength Adenovirus Infectivity Adenovirus Type DNase Digestion 
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  1. 1.
    Corden J, Engelking HM, Pearson GD (1976) Chromatin-like organization of the adenovirus chromosome. Proc Nat Acad Sci 73:401–404PubMedGoogle Scholar
  2. 2.
    Dimmock NJ (1967) Differences between the thermal inactivation of picornaviruses at “high” and “low” temperatures. Virology 31:338–353CrossRefPubMedGoogle Scholar
  3. 3.
    Estes MK, Butel JS (1977) Characterization of a temperature-sensitive mutant of human adenovirus type 7. J Virol 21:1159–1169PubMedGoogle Scholar
  4. 4.
    Galdiero F (1979) Adenovirus aggregation and preservation in extracellular environment. Arch Virol 59:99–105CrossRefPubMedGoogle Scholar
  5. 5.
    Gelderblom H, Bauer H, Frank H, Wigand R (1967) The structure of group II adenoviruses. J Gen Virol 1:553–560PubMedGoogle Scholar
  6. 6.
    Ginoza W (1968) Inactivation of viruses by ionizing radiation and by heat. Methods Virol 4:139–209. K Maramorosch, H Koprowski (eds) Academic Press New York and London 1968Google Scholar
  7. 7.
    Kron I, Buchholz M, Schulz R, Quintenz M, Wigand R (1974) Laboratoriumspraxis bei Adenoviren I. Qualitative und quantitative Immunfluorenszenz. Zentralbakteriol [Orig A] 229:159–170Google Scholar
  8. 8.
    Laver WG, Wrigley NG, Pereira HG (1969) Removal of pentons from particles of adenovirus type 2. Virology 39:599–605CrossRefPubMedGoogle Scholar
  9. 9.
    Prage L, Pettersson U, Höglund S, Lonberg-Holm K, Philipson L (1970) Structural proteins of adenoviruses IV. Sequential degradation of the adenovirus type 2 virion. Virology 42:341–358CrossRefPubMedGoogle Scholar
  10. 10.
    Russell WC, Valentine RC, Pereira HG (1967) The effect of heat on the anatomy of the adenovirus J Gen Virol 1:509–522PubMedGoogle Scholar
  11. 11.
    Wallis C, Melnick JL (1962) Cationic stabilization — a new property of enteroviruses. Virology 16:504–506CrossRefPubMedGoogle Scholar
  12. 12.
    Wallis C, Yang CS, Melnick JL (1962) Effect of cations on thermal inactivation of vaccinia, herpes simplex, and adenoviruses. J Immunol 89:41–46PubMedGoogle Scholar
  13. 13.
    Wigan R, Bachmann P, Brandner G (1981) Stabilization of isometric DNA viruses against thermoinactivation by lowered ionic strength. Arch Virol 69:61–69CrossRefPubMedGoogle Scholar
  14. 14.
    Wigan R, Gelderblom H, özel M (1977) Biological and biophysical characteristics of mouse adenovirus, strain FL. Arch Virol 54:131–142CrossRefPubMedGoogle Scholar
  15. 15.
    Young CSH, Williams JF (1975) Heat-stable variant of human adenovirus type 5: Characterization and use in three-factor crosses. J Virol 15:1168–1175PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Gabriele Sprunck
    • 1
  • R. Wigand
    • 1
  1. 1.Institut für Hygiene und Mikrobiologie, Abteilung für VirologieUniversitÄtsklinikenHomburg (Saar)Federal Republic of Germany

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