Stabilization of adenovirus infectivity against thermoinactivation by lowered ionic strength
- 43 Downloads
The thermostability of adenovirus 5 increases when the ionic strength is decreased before heating by dilution in distilled water (1∶100, heating at 50‡C). A predilution in Eagle's MEM before heating or a previous dialysis against water had no effect on thermostability. The stabilizing effect was found like-wise in crude and purified virus, in heating at 50‡C up to 320 min, from 50‡C to 56‡C, and in dilution from 10−2 to 10−5. The degree of stabilization was similar to cationic stabilization in 2M NaCl. The infectivity of heated adenovirus 5 was not sensitive to DNase digestion. Other adenovirus types were stabilized in a similar manner.
KeywordsIonic Strength Lowered Ionic Strength Adenovirus Infectivity Adenovirus Type DNase Digestion
Unable to display preview. Download preview PDF.
- 6.Ginoza W (1968) Inactivation of viruses by ionizing radiation and by heat. Methods Virol 4:139–209. K Maramorosch, H Koprowski (eds) Academic Press New York and London 1968Google Scholar
- 7.Kron I, Buchholz M, Schulz R, Quintenz M, Wigand R (1974) Laboratoriumspraxis bei Adenoviren I. Qualitative und quantitative Immunfluorenszenz. Zentralbakteriol [Orig A] 229:159–170Google Scholar