Medical Microbiology and Immunology

, Volume 159, Issue 4, pp 317–324 | Cite as

Lysogeny and lysosensitivity inShigella dysenteriae Group of bacteria

III. Serological specificity of receptors of T2 and T4 phages inShigella dysenteriae 7, strain D7
  • Janina Goldhar
  • Emanuel Eylan


Shigella dysenteriae 7 (D7) strain was extracted by using the Westphal water/phenol method and the extract obtained (Aq/Ph 68‡C) was found to have the receptor activity against both T2 and T4 phages.

It was shown by the receptor neutralization test that the examined substance had receptor sites distinctive for T2 and T4 phages. The receptor sites for phage T2 had common antigen specificity with receptor sites of this phage localized inE. coli B in the lipoprotein fraction; the receptor sites of phage T4 had common antigen specificity with receptor sites to phage T4, localized inE. coli B in Aq/Ph 68‡ C extract. It is suggested that in spite of the different localization, the receptors for the same phage in the different bacterial species examined have common serological specificity.


Receptor Activity Bacterial Species Antigen Specificity Receptor Site Neutralization Test 
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  1. Adams, M. H.: The bacteriophages, pp. 443–622. London: Intersci. Publ. 1959Google Scholar
  2. Barber, C., Eylan, E.: Immunochemie des Shigella. V. Immunogenecité et activité biologique des antigens de quatre serotypes de Sh. dysenteriae. Rev. Immunol. (Paris)35, 209–216 (1971)Google Scholar
  3. Beumer, J., Beumer-Jochmans, M. P., Dirx, J., Dekegel, D.: Etat actual des connaissances concernant la nature et la localisation des recepteurs des bacteriophages dans la paroi cellulaire des Shigella et des Escherichia. Bull. Acad. roy. Méd. Belg. Ser. VII,V/9, 749–790 (1965)Google Scholar
  4. Godard, C., Beumer-Jochmans, M. P., Beumer, J.: Apparition de sensibilités aux phages T et a des colicines chez Shigella flexneri F6S survivant a l'infection par un phage Lisbonne. I. Modification des proprietés biologiques des surface. Ann. Inst. Pasteur120, 475–489 (1970)Google Scholar
  5. Goldhar, J., Eylan, E., Goldschmied-Reouven, A.: Lysogeny and lysosensitivity in Shigella dysenteriae group of bacteria. I. Isolation of bacteriophages and receptor substances. Med. Microbiol. Immunol.159, 63–72 (1973)CrossRefPubMedGoogle Scholar
  6. Goldhar, J., Eylan, E., Goldschmied-Reouven, A.: Lysogeny and lysosensitivity in Shigella dysenteriae group of bacteria. II. Receptor sites for temperate phages and serological relationship of indicator strains Sh. boydii 15-7489. Sh. dysenteriae 2 and Sh. dysenteriae 7. Med. Microbiol. Immunol.159, 233–242 (1974)CrossRefPubMedGoogle Scholar
  7. Jesaitis, M. A., Goebel, W. F.: The chemical and antiviral properties of the somatic antigen of Phase II Sh. sonnei. J. exp. Med.96, 409–424 (1952)CrossRefPubMedGoogle Scholar
  8. Lindberg, A. A.: Bacteriophage receptors. Ann. Rev. Microbiol.27, 205–241 (1973)CrossRefGoogle Scholar
  9. Michael, J. G.: The surface antigens and phage receptors in Escherichia coli B (33031). Proc. Soc. exp. Biol. (N.Y.)128, 434–438 (1968)Google Scholar
  10. Rapin, A. M. C., Kalckar, H. H.: The relation of bacteriophage attachment to lipopolysaccharide structure: In Microbial Toxins, vol. IV, pp. 267–307. New York: Academic Press 1971Google Scholar
  11. Welzien, H. U., Jesaitis, M. A.: The nature of the colicin K receptor of Escherichia coli Cullen. J. exp. Med.133, 534–553 (1971)CrossRefPubMedGoogle Scholar
  12. Westphal, O., Jann, K.: Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. In: Methods in Carbohydrate Chemistry, vol. 5, p. 86. New York: Academic Press Inc. 1965Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Janina Goldhar
    • 1
  • Emanuel Eylan
    • 1
  1. 1.Department of Human Microbiology, Medical SchoolTel-Aviv UniversityRamat-Aviv, Tel-AvivIsrael

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