Medical Microbiology and Immunology

, Volume 172, Issue 4, pp 215–222 | Cite as

AStreptococcus mutans immunogen that reacts equally withS. mutans antibody of all serotypes

  • Donald L. Everhart
  • Lisa M. Miglietta
  • Victoria A. Maresca
  • Patricia Kelly-Hatfield


We have studied a possible immunogen fromS. mutans that has the capability of producing antibody toS. mutans which reacts equally well with all serotypes. This immunogen, a ribosomal preparation, is immunogenic in mice, is antigenic with rabbit anti-S. mutans, and is antigenic with the human antibody that also reacts withS. mutans. The human antibody is of the IgG class and S-IgA class.


Human Antibody Ribosomal Preparation Mutans Antibody 
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  1. Arends J (1979) Purification of peroxidase-conjugated antibody for enzyme immunoassay by affinity chromatography on concanavalin A. J Immun Methods 25:171CrossRefGoogle Scholar
  2. Bratthall D, Kohler B (1976)Streptococcus mutans serotypes: some aspects of their identification, distribution, antigenic shifts and relationships to caries. J Dent Res 55: Special Issue C, C15PubMedGoogle Scholar
  3. Bratthall D, Petterson B (1979) Common and unique antigens ofStreptococcus mutans. J Dent Res 55: Special Issue, A60Google Scholar
  4. Campbell DH, Garvey JS, Cremer NE, Sussdorf DH (1977) In: Methods in immunology, 3rd ed, Benjamin, New York, NY, p 522Google Scholar
  5. Everhart DL, Grigsby WR, Carter Jr WH (1972) Evaluation of dental caries experience and salivary immunoglobulins in whole saliva. J Dent Res 51:1487–1491PubMedGoogle Scholar
  6. Everhart DL, Grigsby WR, Carter Jr WH (1973) Human dental caries experience related to age, sex, race and certain salivary properties. J Dent Res 52:242–247PubMedGoogle Scholar
  7. Everhart DL, Schreck M, Seltzer N (1980) Salivary agglutinins tested againstStreptococcus mutans. Caries Res 14:258–264PubMedGoogle Scholar
  8. Gregory RL, Shechmeister IL (1982) Human and cell-mediated responses to a ribosomal preparation fromStreptococcus mutans. Infect Immun 35:1094–1101Google Scholar
  9. Hughes M, Machardy SM, Sheppard AJ, Woods NC (1980) Evidence for an immunological relationship betweenStreptococcus mutans and human cardiac tissue. Infect Immun 27:576–588PubMedGoogle Scholar
  10. Kabat FA, Mayer M (1961) In: Experimental immunochemistry, 2nd ed, Thomas, Springfield, MO, p 708Google Scholar
  11. Kurland CG (1966) The requirements of specific sRNA binding by ribosomes. J Mol Biol 18:90PubMedGoogle Scholar
  12. Lehner T, Cardwell JR, Clarry ED (1967) Immunoglobulins of saliva and serum in dental caries. Lancet i:1294–1297CrossRefGoogle Scholar
  13. Lowry OH, Rosenbrough NJ, Farr AL, Nadel RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265PubMedGoogle Scholar
  14. Orland FJ, Blayney JR, Harrison RW, Reyniers JA, Trexler PC, Ervin RF, Gordon HA, Wagner M (1955) Experimental caries in germ-free rats inoculated with enterocci. J Amer Dent Assoc 50259–272Google Scholar
  15. Orland FJ, Blayney JR, Harrison RW, Reyniers JA, Trexler PC, Ervin RF, Gordon HA, Wagner M, Luckey TD (1954) The use of germ-free animal techniques in the study of experimental dental caries. 1. Basic observations on rats reared free of all microorganisms. J Dent Res 33:147–174PubMedGoogle Scholar
  16. Orstavik P, Bratthall P (1975) Secretion of parotid IgA in relation to gingival inflammation and dental caries experience in man. Archs Oral Biol 20:701–704CrossRefGoogle Scholar
  17. Perch B, Kjems E, Ravn T (1974) Biochemical and serological properties ofStreptococcus mutans from various human and animal sources. Acta Path Microbiol Scand 82:357Google Scholar
  18. Pittman B, Harris PP, Herbert GA, Cherry W (1976) Optimum immunization of rabbits forStreptococcus mutans antiserum and conjugate production and studies of batch immunoabsorption method. J Dent Res 55: Special Issue A, 65–75Google Scholar
  19. van de Rign I, Bleiweis AS, Zabrinskie JB (1976) Antigens inStreptococcus mutans cross-reactive with human heart muscle. J Dent Res 55:59–64PubMedGoogle Scholar
  20. Sato M (1979) Serological properties ofStreptococcus mutans from humans and some animals. J Dent Res 58:461PubMedGoogle Scholar
  21. Snedecor GW, Cochran WG (1967) Statistical methods. 6th ed, The Iowa University Press, IowaGoogle Scholar
  22. Stinson MW, Nisengard RJ, Bergey EJ (1980) Binding of streptococcal antigens to muscles tissue in vitro. Infect Immun 27:604–613PubMedGoogle Scholar
  23. Swenson CL, Johnson W (1976) Humoral immunity toStreptococcus pneumoniae induced by a pneumonococcal ribosomal protein fraction. Infect Immun 14:345–354PubMedGoogle Scholar
  24. Youmans AS, Youmans GP (1965) Immunogenic activity of a ribosomal fraction obtained fromMycobacterium tuberculosis. J Bacteriol 89:1291–1298PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Donald L. Everhart
    • 1
  • Lisa M. Miglietta
    • 1
  • Victoria A. Maresca
    • 1
  • Patricia Kelly-Hatfield
    • 1
  1. 1.Department of MicrobiologyNew York University Dental CenterNew YorkUSA

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