Abstract
A total of 219 urine specimens were Gram stained and inoculated onto 5% sheep blood, eosin methylene blue and chocolate agars. Culture and smear results were evaluated to determine if the Gram stain and chocolate agar culture yielded further meaningful information. Additional information was gained in 42 (19%) of the specimens; however, most of the results were not considered significant. The analysis suggests that a Gram stain and/or chocolate agar culture is not warranted for routine urine culture set-up.
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References
Barry AL, Smith PB, Turck M (1975) Cumitech 2: Laboratory diagnosis of urinary tract infections. Coordinating ed, T Gavan. American Society for Microbiology, Washington, DC
Blaylock BL, Baber S (1980) Urinary tract infection caused byHaemophilus parainfluenzae. Am J Clin Pathol 73:285–287
Cowan ST (1974) Cowan and Steel's, Manual for the identification of medical bacteria, 2nd ed. Cambridge University Press, Cambridge, England
Ewing WH, Martin WJ (1974) Enterobacteriaceae, p 189–221. In Lennette EH, Spaulding EH, Truant JP (eds), Manual of clinical microbiology, 2nd ed. American Society for Microbiology, Washington DC
Hoeprich PD (1960) Culture of the urine. J Lab Clin Med 56:899–907
Kass EH (1956) Asymptomatic infections of the urinary tract. Trans Assoc Am Physicians 69:56–64
Mou TW, Feldman HA (1961) The enumeration and preservation of bacteria in urine. Am J Clin Pathol 35:572–575
Pryles CV (1960) The diagnosis of urinary tract infection. Pediatrics 26:441–451
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Boyd, D.E., Flournoy, D.J. & Hussain Qadri, S.M. An evaluation of the Gram stain and chocolate agar culture as part of a routine urine culture set-up. Med Microbiol Immunol 169, 63–66 (1980). https://doi.org/10.1007/BF02123713
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DOI: https://doi.org/10.1007/BF02123713