Medical Microbiology and Immunology

, Volume 162, Issue 1, pp 63–72 | Cite as

Relationship between measles virus-specific antibody activities and oligoclonal IgG in the central nervous system of patients with subacute sclerosing panencephalitis and multiple sclerosis

  • Erling Norrby
  • Bodvar Vandvik


The association between oligoclonal IgG and measles virus antibodies present in cerebrospinal fluid samples or extracts of brain materials from 9 patients with subacute sclerosing panencephalitis was studied after electrophoretic separation. In all samples there was a good correlation between presence of bands of IgG and occurrence of specific antibody activities. The dominating band of oligoclonal IgG appeared in all cases to represent antibodies against the nucleocapsid antigen. Bands of oligoclonal IgG with a certain antibody activity displayed a tendency to occupy a preferential position in the electropherogram. Antibodies against the hemolysin and the nucleocapsids had a relatively more cathodic position than antibodies against the hemagglutinin.

A clear correlation between bands of oligoclonal IgG and measles virus-specific antibody activity was not found in tests with materials from 4 patients with multiple sclerosis and1 patient with myelopathy of unknown cause. However, a heterogeneous distribution of different antibody activities in separated material suggested that some electrophoretically restricted IgG proteins might carry these activities.


Multiple Sclerosis Measle Clear Correlation Fluid Sample Measle Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Booij, J.: Agar-agar electrophoresis as an aid in cerebrospinal fluid diagnostics. Folia psychiat. neurol.62, 37–43 (1959)Google Scholar
  2. Bücher, T., Matzelf, D., Pette, D.: Papier-elektrophorese vor Liquor Cerebrospinalis. Klin. Wschr.30, 325–330 (1952)CrossRefPubMedGoogle Scholar
  3. Connolly, J. H.: Additional data on measles virus antibody and antigen in subacute sclerosing panencephalitis. Neurology (Minneap.)18, 87–89 (1968)Google Scholar
  4. Cutler, R. W. P., Walters, G. W., Hammarstad, J. P., Merler, E.: Origin of cerebrospinal fluid gamma globulin in subacute sclerosing leukoencephalitis. Arch. Neurol. (Chic.)17, 620–628 (1967)Google Scholar
  5. Kabat, E. A., More, D. H., Landow, H.: An electrophoretic study of the protein components in cerebrospinal fluid and their relationship to the serum proteins. J. clin. Invest.21, 571–577 (1942)Google Scholar
  6. Karcher, D., Matthyssens, G., Lowenthal, A.: Isolation and characterization of IgG globulins in subacute sclerosing panencephalitis. Immunology23, 93–99 (1972)PubMedGoogle Scholar
  7. Laterre, E. C.: Les gammaglobulines du liquide céphalorachidien dans la sclérose en plaques. Acta neurol. belg.66, 305–318 (1966)Google Scholar
  8. Link, H.: Immunoglobulin G and low molecular weight proteins in human cerebrospinal fluid. Chemical and immunological characterization with special reference to multiple sclerosis. Acta neurol. scand.43, Suppl. 28, 1–136 (1967)Google Scholar
  9. Link, H., Norrby, E., Olsson, J.-E.: Immunoglobulin abnormalities and measles antibody response in chronic myelopathy. Arch. Neurol. (Chic.) (in press, 1975)Google Scholar
  10. Link, H., Panelius, M., Salmi, A. A.: Immunoglobulins and measles antibodies in subacute sclerosing panencephalitis. Arch. Neurol. (Chic.)28, 23–30 (1973)Google Scholar
  11. Lowenthal, A.: Agar gel electrophoresis in neurology. Amsterdam: Elsevier 1964Google Scholar
  12. Mancini, G., Vaerman, J. P., Carbonara, A. P., Heremans, J. F.: A single-radial diffusion method for the immunological quantitation of proteins. In: H. Peeters (ed.): XI Colloquium on Protides of the Biological Fluids, pp. 370–373. Amsterdam: Elsevier 1964Google Scholar
  13. Norrby, E., Enders-Ruckle, G., ter Meulen, V.: Qualitative differences in the appearance of antibodies against different structural components of measles virus after immunization with inactivated and live virus. J. infect. Dis.132, 262–269 (1975)PubMedGoogle Scholar
  14. Norrby, E., Gollmar, Y.: Appearance and persistence of antibodies against different virus components after regular measles infections. Infect. Immun.6, 240–247 (1972)PubMedGoogle Scholar
  15. Norrby, E., Gollmar, Y.: Identification of measles virus-specific hemolysis-inhibiting antibodies separate from hemagglutinating-inhibiting antibodies. Inf. Immun.11, 231–239 (1975)Google Scholar
  16. Norrby, E., Link, H., Olsson, J.-E., Panelius, M., Salmi, A., Vandvik, B.: Comparison of antibodies against different viruses in cerebrospinal fluid and serum samples from patients with multiple sclerosis. Infection and Immunity10, 688–694 (1974)PubMedGoogle Scholar
  17. Norrby, E., Link, H., Olsson, J.-E.: Measles virus antibodies in multiple sclerosis. Arch. Neurol. (Chic.)30, 285–292 (1974)Google Scholar
  18. Salmi, A. A., Norrby, E., Panelius, M.: Identification of different measles virus-specific antibodies in the serum and cerebrospinal fluid from patients with subacute sclerosing panencephalitis and multiple sclerosis. Infect. Immun.6, 248–254 (1972)PubMedGoogle Scholar
  19. Schumacher, G. A., Beebe, G., Kubler, R. F., Kurland, L. T., McDowell, F., Nagler, B., Siebley, W. A., Tourtellotte, W. W., Willmon, T. L.: Problems of experimental trials of therapy in multiple sclerosis. Ann. N. Y. Acad. Sci.122, 552–559 (1965)PubMedGoogle Scholar
  20. Sela, M., Mozes, E., Shearer, G. M., Karniely, Y.: Cellular aspects of the inverse relationship between the net charge of immunogens and of antibodies elicited. Proc. nat. Acad. Sci. (Wash.)67, 1288–1293 (1970)Google Scholar
  21. Tourtelotte, W. W., Parker, J. A., Herndon, R. M., Cuadros, C. V.: Subacute sclerosing penencephalitis: brain immunoglobulin-G, measles antibody and albumin. Neurology (Minneap.)18, 117–121 (1968)Google Scholar
  22. Underdown, B. J., Goodfriend, L.: Correlation of charge properties of human reaginic antibodies with charge of the corresponding allergens. J. Immunol.100, 530–533 (1970)Google Scholar
  23. Vandvik, B., Degré, M.: Measles virus antibodies in serum and cerebrospinal fluid in patients with multiple sclerosis and other neurological disorders, with special reference to measles antibody synthesis within the central nervous system. J. neurol. Sci.20, 201–219 (1975)CrossRefGoogle Scholar
  24. Vandvik, B., Norrby, E.: Oligoclonal IgG antibody response in the central nervous system to different measles virus antigens in subacute sclerosing panencephalitis. Proc. nat. Acad. Sci. (Wash.)70, 1060–1063 (1973)Google Scholar
  25. Vandvik, B., Norrby, E., Nordal, H., Degré, M.: Oligoclonal measles virus-specific IgG antibodies isolated by virus immunoabsorption of cerebrospinal fluids, brain extracts, and sera from patients with subacute sclerosing panencephalitis and multiple sclerosis. Scand. J. Immunol. (in press, 1976)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Erling Norrby
    • 1
    • 2
  • Bodvar Vandvik
    • 1
    • 2
  1. 1.The Department of VirologyKarolinska Institute, School of MedicineStockholm
  2. 2.The Institute of Immunology and RheumatologyRikshospitalet

Personalised recommendations