Hepatitis A-virus in cell culture
A test system for the detection of neutralizing antibodies against hepatitis A-virus (anti-HAV-Nt) is presented. The anti-HAV-Nt assay is performed with Frhk-4/R cells and the hepatitis A virus (HAV) strain GBM/Frhk-4/R which has been adapted to these cells. Non-neutralized HAV is demonstrated 14 days after infection of Frhk-4/R cells using a radio-immunoassay for detecting newly grown HAV.
The influence of differing amounts of HAV on the anti-HAV-Nt titre and the effect of variations in incubation time of virus-serum mixtures are described. The time course of anti-HAV-Nt is shown in sera from a hepatitis-A patient which were taken at different stages of the disease. Anti-HAV-Nt is compared with anti-HAV and anti-HAV-IgM. It is shown that anti-HAV-Nt correlates closely with anti-HAV and separated anti-HAV-IgG, but only slightly with anti-HAV-IgM.
The test system presented makes possible the demonstration of neutralizing antibodies against HAV in gamma-globulin preparations and during vaccination studies.
KeywordsCell Culture Hepatitis Incubation Time Vaccination Study
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- 2.Bradley DW, Maynard JE, Hindman SH, Hornbeck CL, Fields HA, McCaustland KA, Cook EH jr. (1977) Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay. J Clin Microbil 5:521–530Google Scholar
- 3.Casey HL (1970) Experience at the National Communicable Disease Center with micro serological techniques for the diagnosis of viral diseases. Health Lab Sci 76:233–242Google Scholar
- 4.Flehmig B (1978) Laboratoriumsdiagnose der Hepatitis A-Infektion. Bundesgesundheitsblatt 17:277–287Google Scholar
- 6.Flehmig B (1981) Hepatitis A-virus in cell culture. II. Growth characteristics of hepatitis A-virus in Frhk-4/R cells. Med Immunol 170:73–81Google Scholar
- 7.Flehmig B, Zahn J (1984) Neuere Ergebnisse in der Hepatitis A-Virus Infektion und Möglichkeiten der Prophylaxe. Ergebnisse aus dem Paul-Ehrlich-Institut, in pressGoogle Scholar
- 11.Kärber G (1931) Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch Pathol Pharmakol 162:480–483Google Scholar
- 16.Lodetti E, De Simone F, Nordelli L (1972) Neutralization tests for foot and mouth disease, equine rhino-, porcine entero-Angeszky-, and rhinopneumonitis viruses. Zentralbl Veterinärmed B 19:848–857Google Scholar
- 18.Mathiesen LR, Feinstone SM, Wong DC, Skinhoej P, Purcell RH (1978) Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid phase radio-immunoassay, immune electron microscopy and immune adherence hemagglutination assay. J Clin Microbiol 7:184–193PubMedGoogle Scholar
- 23.Vesikari T, Vaheri A (1968) Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies. Br Med J I:221–223Google Scholar