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Epstein-Barr virus VCA IgM and EBNA IgG antibodies titered by immunofluorescence in microplates

A semi-automated method based on microtiter system

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Abstract

We describe methods of immunofluorescence in microplates for titration of EBV VCA-IgM and EBNA-IgG antibodies and compare the sensitivity of the methods with glass slides and with microplates, showing the reproducibility of the methods.

Results of VCA-IgM and -IgG, EA and EBNA titers obtained in four groups of patients are given, comprising cases of infectious mononucleosis, renal graft recipients, lymphoproliferative diseases and controls.

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References

  1. Favart AM, Lamy ME, Allemersch D, Burtonboy G, Vanoverschelde J (1978) Epstein-Barr Virus Early Antigen Titer by Immunofluorescence in Microplates. Med Microbiol 166:209–217

    Google Scholar 

  2. Henle G, Henle W (1979) The Virus as Etiologic Agent for Infectious Mononucleosis. In “The Epstein-Barr Virus”, Epstein MA, Achong BG (eds) Springer Berlin Heidelberg New York 298–309

    Google Scholar 

  3. Lamy ME, Favart AM, Burtonboy G, Arana A (1977) Epstein-Barr Virus Viral Capsid Antigen Titer by Immunofluorescence: New semiautomated method based on the Microtiter System. J Clin Microbiol 6:66–71

    PubMed  Google Scholar 

  4. Luka J, Kallin B, Klein G (1979) Induction of the Epstein-Barr Virus (EBV) cycle in latently infected cells by n-Butyrate. Virology 93:228–231

    Article  Google Scholar 

  5. Reedman BM, Klein G (1973) Cellular Localization of an Epstein-Barr Virus (EBV) — associated complement — fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer 11:499–520

    PubMed  Google Scholar 

  6. Schmitz H, Scherer M (1972) IgM antibodies to Epstein-Barr Virus in infectious mononucleosis. Archiv für die gesamte Virusforschung 37:332–339

    Article  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Virologie, Universite Catholique de Louvain en Woluwé, Bruxelles, Belgique

    M. E. Lamy, A. M. Favart, M. F. Leclercq, M. Segas, M. Mendez, C. Lhoir, C. Cornu & G. Burtonboy

Authors
  1. M. E. Lamy
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  2. A. M. Favart
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  3. M. F. Leclercq
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  4. M. Segas
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  5. M. Mendez
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  6. C. Lhoir
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  7. C. Cornu
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  8. G. Burtonboy
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Lamy, M.E., Favart, A.M., Leclercq, M.F. et al. Epstein-Barr virus VCA IgM and EBNA IgG antibodies titered by immunofluorescence in microplates. Med Microbiol Immunol 170, 247–253 (1982). https://doi.org/10.1007/BF02123315

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  • DOI: https://doi.org/10.1007/BF02123315

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