Abstract
Native flagella of tenSalmonella serotypes were shown to possess serotype-specific antigenic determinants as well as a multiple of common antigenic determinants, which varied in concentration. Each common antigenic determinant was shared by certain, but not all, serotypes. This has been demonstrated from agglutination and radioimmunometric assay (RIMA) results, using ten antisera raised in rabbits against purified polymeric flagellins from tenSalmonella serotypes as immunogens. The minimum detectable populations of salmonellae, as determined by RIMA varied considerably, due to variation in concentrations (and possibly types) of common antigenic determinants. However, the results demonstrated the feasibility of utilizing RIMA for the qualitative detection of salmonellae, using a mixture of only the ten antisera and125I-labelled protein A as a general tracer. In this way, 77 different salmonellae were detected in less than 8 h after culturing in selective broth. The RIMA developed was specific for salmonellae and showed no cross-reactions with high populations of other members within the familiyEnterobacteriaceae.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aksoycan N, Mercangoz F, Saganak I (1979a) The antigenic relationship betweenKloeckera andSalmonella cbolerae-suis O antigen. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg I Abt A245:67–70
Aksoycan N, Mercangoz F, Wells G (1979b) The O antigenic relationship ofKloeckera africana, Salmonella aberdeen andEscherichia coli 0 75. Ann Microbiol 130B:29–31
Aksoycan N, Saganak I (1980) The antigenic relationship betweenCandida andSalmonella 0:6, 0:7antigens. Zntralbl Bakteriol Mikrobiol Hyg I Abt A247:406–409
Clark DJ, MaalØe O. (1967) DNA replication and the division cycle inEscherichia coli. J Mol Biol 23:99–112
Dorval G, Welsh Kl, Wigzell H (1975) A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein A fromStaphylococcus aureus. J Immunol Methods 7:237–249
Edwards PR, Ewing WH (1972) The genusSalmonella. In: Identification of Entero-bacteriacea. Burgess, Minneapolis, Minnesota, pp 184–189
Fenstein A, Munn EA (1966) An electron microscopic study of the interaction of macroglobulin (IgM) antibodies with bacterial flagella and of the binding of complement. J Physiol 186:64P.
Guesdon J, Ternyck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139
Harris CC, Yolken RH, Krokan H, Hsu IC (1979) Ultrasensitive enzymatic radioimmunoassay: Application to detection of cholera toxin and rotavirus. Proc Natl Acad Sci 76:5336–5339
Hsu IC, Poirier MC, Yuspa SH, Yolken RH, Harris CC (1980) Ultrasensitive enzymatic radioimmunoassay (USERIA) detects femtomoles of acetylaminofluorene-DNA adducts. Carcinogenesis 1:455–458
Hsu IC, Poirier MC, Yuspa SH, Grunberger D, Weinstein IB, Yolken RH, Harris CC (1981) Measurement of benzo (a) pyrene-DNA adducts by enzyme immunoassays and radioimmunoassay. Cancer Research 41:1091–1095
Kauffmann F (1975) The Kauffmann-White schema. In: Classification of bacteria. Scandinavian University Books, Munskgaard-Copenhagen, pp 61–63
Kendall C, Ionescu-Matiu I, Drecsman GR (1983) Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunpsorbent assay (ELISA). J Immunol Methods 56:329–339
Kennedy JF, Barker SA, Humphreys JD (1976) Microbial cells living immobilized on metal hydroxides. Nature 261:242–244
Langman RE (1972) The occurrence of antigenic determinants common to flagella of differentSalmonella strains. Eur J Immunol 2:582–586
Langone JJ (1980a)125I-labelled protein A as a general tracer in immunoassays: Suitability of goat and sheep antibodies. J Immunol Methods 34:93–106
Langone JJ (1980b)125I-labelled protein A: Reactivity with IgG and use as a tracer in radioimmunoassay. In: van Vunakis H, Langone JJ (eds) Methods in Enzymol. Academic Press, London, New York, Vol. 70, pp 356–375
Orskov I, Orskov F, Jann B, Jann K (1977) Serology, chemistry and genetics of O and K antigens ofEscherichia coli. Bacteriol Rev 41:667–710
Rodbard D, Rayford P, Cooper JA, Ross GT (1968) Statistical quality control of radio-immunoassays. J Clin Endocrinol 28:1412–1418
Vaitukaitis J, Robbins JB, Nieschlag E, Ross GT (1971) A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol 33:988–991
Yalow RS (1980) Radioimmunoassay. Ann Rev Biophys. Bioeng. 9:327–345
Yolken RH, Leister FJ, Whitcomb LS, Santosham M (1983) Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labelled antibody and peroxidase biotin-avidin complex. J Immunol Methods 56:319–327
Zettner A (1973) Principles of competitive binding assays (saturation analysis). I. Equilibrium techniques. Clin Chem 19:699–705
Zettner A, Duly PE (1974) Principles of competitive binding assays (saturation analysis). II. Sequential saturation. Clin Chem 20:5–14
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ibrahim, G.F., Fleet, G.H., Lyons, M.J. et al. Immunological relationships betweenSalmonella flagella and their potential application for salmonellae detection by immunoassay. Med Microbiol Immunol 174, 87–99 (1985). https://doi.org/10.1007/BF02123230
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02123230