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Β2-microglobulin secretion from lymphoid cells: A study at the cellular level

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Abstract

A protein plaque assay was developed for the detection of human lymphocytes secretingΒ 2-microglobulin. The development of plaques required production ofΒ 2-microglobulin by viable cells at an estimated rate exceeding 25 molecules per second. It has previously been suggested thatΒ 2-microglobulin secretion is restricted to certain lymphoid cell subpopulations. However, both T- and B-cells were shown to form plaques in normal donors and immunodeficient patients after activation with various mitogens.

A close correlation betweenΒ 2-microglobulin production and DNA synthesis was evident. However, secretion of immunoglobulin did not correlate with the proportion of cells secretingΒ 2-microglobulin.

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Authors and Affiliations

  1. Department of Immunobiology, Wallenberglaboratory, Lilla Frescati, S-104 05, Stockholm 50, Sweden

    L. Hammarström & C. I. E. Smith

  2. Department of Clinical Immunology, Huddinge Hospital, S-141 86, Huddinge, Sweden

    L. Hammarström & C. I. E. Smith

Authors
  1. L. Hammarström
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  2. C. I. E. Smith
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Hammarström, L., Smith, C.I.E. Β2-microglobulin secretion from lymphoid cells: A study at the cellular level. Med Microbiol Immunol 174, 59–66 (1985). https://doi.org/10.1007/BF02123226

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  • DOI: https://doi.org/10.1007/BF02123226

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