Abstract
The in vitro stimulation of lymphocytes from 15 healthy adult donors by 5 different strains of CMV resulted in a positive stimulation ratio of lymphocytes from CMV seropositive donors with each of the 5 different strains. Differences in the absolute strength of the response was observed among the 5 strains for each individual, but we conclude that the use of antigen prepared with 1 strain of CMV is sufficient to obtain a positive or negative result. A maximum value for the in vitro lymphocyte stimulation test, however, will only be obtained using different antigen concentrations from several different CMV strains simultaneously.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agatsuma Y, Fitzpatrick R, Kaul R, Kohli R, Paves AV, Ogra PL (1979) Cell-mediated immunity to cytomegalovirus in immunocompromised patients: Effect of radiation or chemotherapy. Infect Immun 26:217–224
Beutner KR, Morag A, Deibel R, Morag B, Raiken D, Ogra PL (1978) Strain-specific local and systemic cell-mediated immune responses to cytomegalovirus in humans. Infect Immun 20:82–87
Gärtner L, Oberender H, Kunkel M (1982) Application of different strains for determining IgM antibodies against cytomegalovirus-induced late antigen. Arch Virol 72:133–135
Haahr S, Rassmussen L, Merigan TC (1976) Lymphocyte transformation and interferon production in human mononuclear cell microcultures for assay of cellular immunity to herpes simplex virus. Infect Immun 14:47–54
Krech U, Jung M, Sonnabend W (1970) A study of complement-fixing, immunofluorescence and neutralizing antibodies in human cytomegalovirus infections. Z Immun-Forsch 141:411–429
MØller-Larsen A, Andersen HK, Heron I, Sarov I (1976) In vitro stimulation of human lymphocytes by purified cytomegalovirus. Intervirology 6:249–252
Pollard RB, Rand KH, Arvin AM, Merigan TC (1978) Cell-mediated immunity to cytomegalovirus infection in normal subjects and cardiac transplant patients. J Infect 137:541–549
Quinnan GV, Ennis FA (1980) Cell-mediated immunity in cytomegalovirus infections — a review. Comp Immun Microbiol Infect Dis 3:283–290
Reynolds DW, Dean PH, Pass RF, Alford CA (1979) Specific cell-mediated immunity in children with congenital and neonatal cytomegalovirus infection and their mothers. J Infect Dis 140:493–499
Sarov I, MØller-Larsen A, Heron I, Andersen HK (1978) Stimulation of human lymphocytes by cytomegalovirions and dense bodies. Med Microbiol Immunol 166:81–89
Schirm J, Rosenhorst HW, The TH (1980) Comparison of in vitro lymphocyte proliferations induced by cytomegalovirus-infected human fibroblasts and cell-free cytomegalovirus. Infect Immun 30:621–627
Starr SE, Dalton B, Garrabrant T, Pauker K, Plotkin SA (1980) Lymphocyte blastogenesis and interferon production in adult human lymphocyte cultures stimulated with cytomegalovirus antigens. Infect Immun 30:17–22
Ten Napel Chr HH, The TH, Bijker J, de Gast GC, Langenhuysen MMAC (1977) Cytomegalovirus-directed lymphocyte reactivity in healthy adults tested by a CMV-induced lymphocyte transformation test. Clin Exp Immunol 29:52–60
Ten Napel Chr HH, The TH (1980) Acute cytomegalovirus infection and the host immune response. I. Development and maintenance of cytomegalovirus (CMV) induced in vitro lymphocyte reactivity and its relationship to the production of CMV antibodies. Clin Exp Immunol 39:263–271
Wilhelm J, Longthorne R (1980) An enhancement by CMV seropositive serum or plasma of lymphocyte response in an in vitro CMV-specific lymphocyte transformation test. Immunol Letters 1:287–291
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Schmidt on his 60th birthday
Rights and permissions
About this article
Cite this article
Gärtner, L., Wilhelm, J.A. & Czieschnek, R. In vitro stimulation of lymphocytes by different strains of cytomegalovirus. Med Microbiol Immunol 171, 53–57 (1982). https://doi.org/10.1007/BF02122707
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02122707