Medical Microbiology and Immunology

, Volume 171, Issue 1, pp 23–32 | Cite as

Serological grouping of streptococci: A collective evaluation in six laboratories of three rapid methods

  • R. Guinet
  • J. André
  • G. Barbé
  • M. Boude
  • G. Carret
  • A. -M. Freydière
  • Y. Gille
  • J. -P. Marcel
  • M. -A. Mazoyer
Article

Abstract

Three serological methods for streptococci grouping: latex agglutination after pronase extraction (LAP), latex agglutination after nitrous acid extraction (LAN) and counterimmunoelectrophoresis after nitrous acid extraction (CIEN) were evaluated in six laboratories with 310 strains of streptococci. To reduce the possible influence of a particular laboratory, each serological procedure was performed in two different laboratories with identical protocols and strains. Between the three procedures, a total agreement of 81.3 % with six identical results was obtained. Since only one discordant result was obtained in 12.2 % (CIEN 4.8 % — LAN 5.8 % — LAP 1.6 %) and two discordant results with two different serological methods in 2.6 %, it could be concluded that agreement between the three procedures was 96 %. For B (99 strains tested) and D (100 strains tested) groups, total agreements of respectively 95 % and 92 % were achieved. In conclusion, LAP, LAN, and CIEN gave equivalent results for streptococci grouping, but LAN was the most simple needing no heating, centrifugation, or electrophoresis equipment and lacking cross-reactivity of group C antibody.

Keywords

Nitrous Acid Identical Result Acid Extraction Rapid Method Equivalent Result 

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References

  1. Arvilommi H, Uurasmaa O, Nurkkala A (1978) Rapid identification of group A, B, C and G beta-hemolytic streptococci by a modification of the co-agglutination technique. Comparison of results obtained by co-agglutination, fluorescent antibody test, counterimmunoelectrophoresis and precipitin technique. Acta path microbiol scand, Sect B 86:107Google Scholar
  2. Carlson JR, McCarthy LR (1979) Modified co-agglutination procedure for the serological grouping of streptococci. J clin Microbiol 9:329PubMedGoogle Scholar
  3. Christensen P, Kahlmeter G, Jonsson S, Kronvall G (1973) New method for the serological grouping of streptococci with specific antibodies absorbed to protein A containing staphylococci. Infect Immun 7:881PubMedGoogle Scholar
  4. Croix JC, Bajolle F, Dalle M (1975) Application de l'électroimmunodiffusion au groupage des streptocoques. Etude de 332 souches d'origine hospitalière. Ann Biol clin 33:163Google Scholar
  5. Dajani AS (1973) Rapid identification of beta-hemolytic streptococci by counterimmunoelectrophoresis. J Immunol 110:1702PubMedGoogle Scholar
  6. Ederer GM, Herman MM, Burce R, Matsen JM, Chapman SS (1972) Rapid extraction method with pronase B for grouping beta-hemolytic streptococci. Appl Microbiol 23:285PubMedGoogle Scholar
  7. Edwards EA, Larson GL (1973) Serological grouping of hemolytic streptococci by immunoelectrophoresis. Appl Microbiol 25:899Google Scholar
  8. Efstratiou A, Maxted WR (1979a) Agglutination and co-agglutination methods for grouping streptococci. In: Parker MT (ed) Pathogenic Streptococci, Redboods, Chertsey, Surrey, p 254–255Google Scholar
  9. Efstratiou A, Maxted WR (1979b) Serological grouping of streptococci by slide agglutination. J clin Path 32:1228PubMedGoogle Scholar
  10. El Kholy A, Facklam RR, Sabri G, Rotta J (1978) Serological identification of group A streptococci from throat scrapings before culture. J clin Microbiol 8:725PubMedGoogle Scholar
  11. El Kholy A, Wannamaker LW, Krause RM (1974) Simplified extraction procedure for serological grouping of beta-hemolytic streptococci. Brit J exp Path 19:130Google Scholar
  12. Facklam RR, Cooksey RC, Wortham EC (1979) Evaluation of commercial Latex Agglutination Reagents for grouping streptococci. J clin Microbiol 10:641PubMedGoogle Scholar
  13. Farrar JL, Paull A (1979) Grouping of streptococci by means of latex particles coated with antibody. In: Parker MT (ed) Pathogenic Streptococci, Redbooks, Chertsey, Surrey, p 256Google Scholar
  14. Fuller AT (1938) The formamid method for the extraction of polysaccharide from beta-hemolytic streptococci. Brit J Path 19:131Google Scholar
  15. Guinet R (1977) Groupage des streptocoques par électrosynérèse. Rev Inst Pasteur Lyon 10:207Google Scholar
  16. Guinet R (1980) Groupage rapide des streptocoques par agglutination latex après extraction à l'acide nitreux. Ann Biol clin 38:15Google Scholar
  17. Guinet R, Andre J, Gille Y, Guillermet FN (1977) Détection rapide avec identification spécifique des streptocoques par électrosynérèse (électroimmunodiffusion). Rev Inst Pasteur Lyon 10:25Google Scholar
  18. Guinet R, Boude M, Guillermet FN (1979) Groupage des streptocoques par agglutination latex: comparaison avec l'électrosynérèse et la précipitation selon Lancefield. Rev Inst Pasteur Lyon 12:403Google Scholar
  19. Laban P, Chastel Cl (1976) Technique simple et rapide de groupage des streptocoques. Ann Biol clin 34:229Google Scholar
  20. Lancefield R (1933) A serological differenciation of human and other groups of hemolytic streptococci. J exp Med 57:571Google Scholar
  21. Lue YA, Howit IP, Ellner P (1978) Rapid grouping of beta-hemolytic streptococci by latex agglutination. J clin Microbiol 8:326PubMedGoogle Scholar
  22. Maxted WR (1948) Preparation of streptococcal extracts for Lancefield grouping. Lancet 11:255Google Scholar
  23. Rantz LA, Randall E (1955) Use of autoclaved extracts of hemolytic streptococci for serological grouping. Standford med Bull 13:290Google Scholar
  24. Rosendal K (1956) Grouping of hemolytic streptococci belonging to group A, C and G. A comparison between results obtained by precipitation and by slide agglutination. Act path microbiol scand, Sect B 39:127Google Scholar
  25. Slifkin M, Interval G (1980) Serogrouping single colonies of beta-hemolytic streptococci from primary throat culture plates with nitrous acid extraction and phadebact streptococcal reagents. J clin Microbiol 12:531Google Scholar
  26. Slifkin M, Pouchet-Melvin GR (1980) Evaluation of three commercially available test products for serogrouping beta-hemolytic streptococci. J clin Microbiol 11:249PubMedGoogle Scholar
  27. Swanson J, Hsu KC, Gotschlich ED (1969) Electron microscopic studies on streptococci I.M antigen. J exp Med 130:1063PubMedGoogle Scholar
  28. Swift HF, Wilson AT, Lancefield RC (1943) Typing group A hemolytic streptococci by M precipitin reactions in capillary lubes. J exp Med 78:127Google Scholar
  29. Wadstrom T, Nord CE, Lindberg AA, Molby R (1974) Rapid grouping of streptococci by immunoelectrophoresis. Med Microbiol 159:191Google Scholar
  30. Wautelet J, Wauters G, Bruynoghe G (1975) Détermination du groupe des streptocoques au moyen d'anticorps fixés à la protéine A staphylococcique. Ann Microbiol (Inst Pasteur) 126 B:251Google Scholar
  31. Watson BK, Moellering RC, Kunz LF (1975) Identification of streptococci: use of lysozyme andStreptomyces albus filtrate in the preparation of extracts for Lancefield grouping. J clin Microbiol 1:274PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • R. Guinet
    • 1
  • J. André
    • 1
  • G. Barbé
    • 1
  • M. Boude
    • 1
  • G. Carret
    • 1
  • A. -M. Freydière
    • 1
  • Y. Gille
    • 1
  • J. -P. Marcel
    • 1
  • M. -A. Mazoyer
    • 1
  1. 1.G.E.R.M. (Groupe d'études et de recherches en microbiologie)LyonFrance

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