Advertisement

Medical Microbiology and Immunology

, Volume 159, Issue 1, pp 73–82 | Cite as

Neutralization of infectious ME (Maus-Elberfeld) virus-antibody complexes by anti-γ-globulin antibody

  • Josef Cihak
Article

Abstract

Neutralization of MB (Maus-Elberfeld) virus by rabbit anti-ME virus antiserum has been studied by the plaque reduction method. A certain amount of virus scored as infectious even after prolonged incubation with antibody in excess. This persistent virus fraction was readily neutralized by sheep anti-rabbitγ-globulin antiserum, complement, and unheated calf serum. The results of these experiments are interpreted to mean that the persistent fraction of MB virus consists of virus which has retained its infectivity in spite of attached antibody. The persistent virus was neutralized by anti-γ-globulin antiserum even after adsorption of the virus to susceptible cells, indicating that it was still complexed with antibody. Virus free of antibody or virus aggregates could not be demonstrated in the persistent fraction.

Keywords

Calf Serum Reduction Method Prolonged Incubation Susceptible Cell Persistent Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ashe, W. K., Notkins, A. L.: Neutralization of an infectious herpes simplex virus-antibody complex by anti-γ-globulin. Proc. nat. Acad. Sci. (Wash.)56, 447–451 (1966)Google Scholar
  2. 2.
    Ashe, W. K., Notkins, A. L.: Kinetics of sensitization of herpes simplex virus and its relationship to the reduction in the neutralization rate constant. Virology33, 613–617 (1967)Google Scholar
  3. 3.
    Baughman, R. H., Fenters, J. D., Marquis, G. S., Jr., Holper, J. C.: Effect of complement and viral filtration on the neutralization of respiratory syncytial virus. Appl. Micobiol.16, 1076–1080 (1968)Google Scholar
  4. 4.
    Daniels, Ch. A., Borsos, T., Rapp, H. J., Snyderman, R., Notkins, A. L.: Neutralization of sensitized virus by the fourth component of complement. Science165, 508–509 (1969)PubMedGoogle Scholar
  5. 5.
    Daniels, Ch., Borsos, T., Rapp, H. J., Snyderman, R., Notkins, A. L.: Neutralization of sensitized virus by purified components of complement. Proc. nat. Acad. Sci (Wash.)65, 528–535 (1970)Google Scholar
  6. 6.
    Dulbecco, R., Vogt, M., Strickland, A. G. R.: A study of the basic aspects of neutralization of two animal viruses, Western equine encephalitis virus and poliomyelitis virus. Virology2, 162–205 (1956)PubMedGoogle Scholar
  7. 7.
    Fahey, J. L.: Chromatographic separation of immunoglobulins. In: C. A. Williams and M. W. Chase: Methods in immunology and immunochemistry, Vol. I, pp. 321–332. New York-London: Academic Press 1967Google Scholar
  8. 8.
    Fazekas de St. Groth, S., Watson, G. S., Reid, A. F.: The neutralization of animal viruses. I. A model of virus-antibody interaction. J. Immunol.80, 215–224 (1958)PubMedGoogle Scholar
  9. 9.
    Fiala, M.: A study of the neutralization of rhinoviruses by means of plaquereduction test. J. Immunol.103, 107–113 (1969)PubMedGoogle Scholar
  10. 10.
    Granoff, A.: The interaction of Newcastle disease virus and neutralizing antibody. Virology25, 38–47 (1965)PubMedGoogle Scholar
  11. 11.
    Groc, W.: Die zentripetal-radiale Immunodiffusion. Clin. chim. Acta30, 365–368 (1970)PubMedGoogle Scholar
  12. 12.
    Hahon, N.: Neutralization of residual infectivity of Venezuelan equine encephalomyelitis virus by anti-gamma globulin. J. gen. Virol.6, 361–372 (1970)PubMedGoogle Scholar
  13. 13.
    Hampar, B., Notkins, A. L., Mage, M., Keehn, M. A.: Heterogeneity in the properties of 7 S and 19 S rabbit-neutralizing antibodies to herpes simplex virus. J. Immunol.100, 586–593 (1968)PubMedGoogle Scholar
  14. 14.
    Hashimoto, N., Prince, A. M.: Kinetic studies on the neutralization reaction between Japanese encephalitis virus and antiserum. Virology19, 261–272 (1963)PubMedGoogle Scholar
  15. 15.
    Kjellén, L.: Density gradient centrifugations of adenovirus-antibody complexes. Arch. ges. Virusforsch.17, 398–408 (1965)PubMedGoogle Scholar
  16. 16.
    Lafferty, K. J.: The interaction between virus and antibody. I. Kinetic studies. Virology21, 61–75 (1963)PubMedGoogle Scholar
  17. 17.
    Lafferty, K. J.: The relationship between molecular charge and the biological activity of antibody directed against rabbitpox virus. Virology25, 591–602 (1965)PubMedGoogle Scholar
  18. 18.
    Linscott, W. D., Levinson, W. E.: Complement components required for virus neutralization by early immunoglobulin antibody. Proc. nat. Acad. Sci. (Wash.)64, 520–527 (1969)Google Scholar
  19. 19.
    Majer, M., Link, F.: Studies on the non-neutralizable fraction of vaccinia virus. Clin. exp. Immunol.7, 283–291 (1970)PubMedGoogle Scholar
  20. 20.
    Notkins, A. L., Mahar, S., Scheele, Ch., Goffman, J.: Infectious virus-antibody complex in the blood of chronically infected mice. J. exp. Med.124, 81–97 (1966)PubMedGoogle Scholar
  21. 21.
    Notkins, A. L., Mage, M., Ashe, W. K., Mahar, S.: Neutralization of sensitized lactic dehydrogenase virus by anti-γ-globulin. J. Immunol.100, 314–320 (1968)Google Scholar
  22. 22.
    Philipson, L.: Interaction between poliovirus and immunoglobulins. II. Basic aspects of virus-antibody interaction. Virology28, 35–46 (1966)PubMedGoogle Scholar
  23. 23.
    Thormar, H.: Neutralization of visna virus by antisera from sheep. J. Immunol.90, 185–192 (1963)PubMedGoogle Scholar
  24. 24.
    Wallis, C., Melnick, J. L.: Virus aggregation as the cause of the non-neutralizable persistent fraction. J. Virol.1, 478–488 (1967)Google Scholar
  25. 25.
    Wallis, C., Melnick, J. L.: Herpesvirus neutralization: induction of the persistent fraction by insufficient antibody. Virology42, 128–137 (1970)PubMedGoogle Scholar
  26. 26.
    Westaway, E. G.: Antibody responses in rabbits to the group B arbovirus Kunjin: serologic activity of the fractionated immunoglobulins in homologous and heterologous reactions. J. Immunol.100, 569–580 (1968)PubMedGoogle Scholar
  27. 27.
    Zalan, E., Borman, E., Labzoffsky, N. A.: Infectious influenza virus-antibody complex. Arch. ges. Virusforsch.34, 209–213 (1971)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Josef Cihak
    • 1
  1. 1.Heinrich-Pette-Institut für experimentelle Virologie und Immunologie an der Universität HamburgDeutschland

Personalised recommendations