Neutralization of infectious ME (Maus-Elberfeld) virus-antibody complexes by anti-γ-globulin antibody
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Neutralization of MB (Maus-Elberfeld) virus by rabbit anti-ME virus antiserum has been studied by the plaque reduction method. A certain amount of virus scored as infectious even after prolonged incubation with antibody in excess. This persistent virus fraction was readily neutralized by sheep anti-rabbitγ-globulin antiserum, complement, and unheated calf serum. The results of these experiments are interpreted to mean that the persistent fraction of MB virus consists of virus which has retained its infectivity in spite of attached antibody. The persistent virus was neutralized by anti-γ-globulin antiserum even after adsorption of the virus to susceptible cells, indicating that it was still complexed with antibody. Virus free of antibody or virus aggregates could not be demonstrated in the persistent fraction.
KeywordsCalf Serum Reduction Method Prolonged Incubation Susceptible Cell Persistent Virus
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