Abstract
In solid phase indirect radioimmunoassay (IRIA) antiviral immunoglobulins (Ig) bind specifically to the viral antigen fixed onto wells of microtiter plates. Radioactively labeled (125I) anti-Ig can be used to detect these antiviral antibodies. The sensitivity of the IRIA depends on the amount of antigen in the microtiter wells and the concentration of the125I-labeled anti-Ig used. To standardize the assay, a method of antigen titration in the IRIA was devised, using parainfluenza type 1 viruses as antigens.
The IRIA provides a means to titrate viral antigens with different biologic activities and antibodies against them. A binding inhibition test (BIT) based on IRIA allows the antigenic analysis of different virus strains, as is demonstrated for two closely related parainfluenza type 1 virus strains.
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Supported by Deutsche Forschungsgemeinschaft, Az.: Me 270/14
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Yung, L.L.L., Loh, W. & ter Meulen, V. Solid phase indirect radioimmunoassay: Standardization and applications in viral serology. Med Microbiol Immunol 163, 111–123 (1977). https://doi.org/10.1007/BF02121826
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DOI: https://doi.org/10.1007/BF02121826