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Medical Microbiology and Immunology

, Volume 158, Issue 4, pp 259–266 | Cite as

New approaches to the isolation of mycoplasmas

  • P. -A. Mårdh
  • D. Taylor-Robinson
Article

Abstract

Homogenates of rodent and human tissues after storage at 4°C for 72 h killedMycoplasma pulmonis. Lysolecithin also killed the organisms. Several ways of overcoming this mycoplasmacidal activity were investigated. Of these, success was achieved by the addition of ammonium reineckate or lysophospholipase to medium containing lysolecithin; mycoplasmacidal activity was abolished. These substances were then used in attempts to overcome the inhibitory activity of hamster lung tissue onM. pneumoniae organisms. The organisms were isolated from suspensions of the tissue that otherwise inhibited their growth. These results lend support to the idea that lysolecithin is important for the mycoplasmacidal activity of tissue homogenates and indicate the potential value in mycoplasma isolations of the commercially available compound, ammonium reineckate.

Keywords

Ammonium Inhibitory Activity Lung Tissue Human Tissue Tissue Homogenate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • P. -A. Mårdh
    • 1
    • 2
  • D. Taylor-Robinson
    • 1
  1. 1.M.R.C. Clinical Research CentreHarrowEngland
  2. 2.Institute of Medical MicrobiologyUniversity of LundSweden

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