New approaches to the isolation of mycoplasmas
Homogenates of rodent and human tissues after storage at 4°C for 72 h killedMycoplasma pulmonis. Lysolecithin also killed the organisms. Several ways of overcoming this mycoplasmacidal activity were investigated. Of these, success was achieved by the addition of ammonium reineckate or lysophospholipase to medium containing lysolecithin; mycoplasmacidal activity was abolished. These substances were then used in attempts to overcome the inhibitory activity of hamster lung tissue onM. pneumoniae organisms. The organisms were isolated from suspensions of the tissue that otherwise inhibited their growth. These results lend support to the idea that lysolecithin is important for the mycoplasmacidal activity of tissue homogenates and indicate the potential value in mycoplasma isolations of the commercially available compound, ammonium reineckate.
KeywordsAmmonium Inhibitory Activity Lung Tissue Human Tissue Tissue Homogenate
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