Medical Microbiology and Immunology

, Volume 158, Issue 4, pp 259–266 | Cite as

New approaches to the isolation of mycoplasmas

  • P. -A. Mårdh
  • D. Taylor-Robinson


Homogenates of rodent and human tissues after storage at 4°C for 72 h killedMycoplasma pulmonis. Lysolecithin also killed the organisms. Several ways of overcoming this mycoplasmacidal activity were investigated. Of these, success was achieved by the addition of ammonium reineckate or lysophospholipase to medium containing lysolecithin; mycoplasmacidal activity was abolished. These substances were then used in attempts to overcome the inhibitory activity of hamster lung tissue onM. pneumoniae organisms. The organisms were isolated from suspensions of the tissue that otherwise inhibited their growth. These results lend support to the idea that lysolecithin is important for the mycoplasmacidal activity of tissue homogenates and indicate the potential value in mycoplasma isolations of the commercially available compound, ammonium reineckate.


Ammonium Inhibitory Activity Lung Tissue Human Tissue Tissue Homogenate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arnesjö, B.: In: Pancreatic phospholipase, p. 33. Lund: Studentlitteratur 1968.Google Scholar
  2. Dajani, A. S., Ayoub, E. M.: Mycoplasmacidal effect of polymorphonuclear leukocyte extract. J. Immunol.102, 698 (1969).PubMedGoogle Scholar
  3. Kaklamanis, E., Stavropoulos, K., Thomas, L.: The mycoplasmacidal action of homogenates of normal tissue. In: S. Madoff (Ed.): Mycoplasma and thel forms of bacteria, pp. 27–35. London: Gordon and Beach Sci. Publ. 1971.Google Scholar
  4. Kaklamanis, E., Thomas, L., Stavropoulos, K., Borman, I., Boshwitz, C.: Mycoplasmacidal action of normal tissue extracts. Nature (Lond.)221, 860 (1969).Google Scholar
  5. Manchee, R. J., Taylor-Robinson, D.: Haemadsorption and haemagglutination by mycoplasmas. J. gen. Microbiol.50, 465 (1968).PubMedGoogle Scholar
  6. Mårdh, P.-A., Taylor-Robinson, D.: The differential effect of lysolecithin on mycoplasmas and acholeplasmas. Med. Microbiol. Immunol.158, 219–225 (1973).PubMedGoogle Scholar
  7. Metcalfe, J. C., Marlow, H. F., Burgen, A. S. V.: Immuno-adsorbents of high capacity. Nature (Lond.)209, 1142 (1966).Google Scholar
  8. Taylor-Robinson, D., Denny, F. W., Thompson, G. W., Allison, A. C., Mårdh, P.-A.: Isolation of mycoplasmas from lungs by a perfusion technique. Med. Microbiol. Immunol.158, 9 (1972).PubMedGoogle Scholar
  9. Taylor-Robinson, D., Purcell, R. H.: Mycoplasmas of the human urogenital tract and oropharynx and their possible role in disease: a review with some recent observations. Proc. roy. Soc. Med.59, 1112 (1966).PubMedGoogle Scholar
  10. Tully, J. G., Rask-Nielsen, R.: Mycoplasma in leukemic and nonleukemic mice. Ann. N.Y. Acad. Sci.143, 345 (1967).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • P. -A. Mårdh
    • 1
    • 2
  • D. Taylor-Robinson
    • 1
  1. 1.M.R.C. Clinical Research CentreHarrowEngland
  2. 2.Institute of Medical MicrobiologyUniversity of LundSweden

Personalised recommendations