Medical Microbiology and Immunology

, Volume 162, Issue 2, pp 81–87 | Cite as

Detection of cytotoxic lymphoid spleen cells from STU-mice with Moloney sarcoma by a3H-proline microcytotoxicity assay

  • Emilie Weiland
  • Manfred Mussgay


A microcytotoxicity assay with3H-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from STU inbred mice inoculated with Moloney sarcoma virus (MSV-M) or ascitic MSV-M tumor cells. The target cell line was derived from ascitic MSV-M tumor cells. With regard to the specificity of the assay nonimmune spleen cells displayed no or only a weak cytotoxicity against these cells, and this was also the case when3H-proline-labeled secondary cultures of syngeneic mouse embryo cells were exposed to both sensitized and nonimmune spleen cells. The time-course pattern of the development of cytotoxic lymphoid spleen cells in STU mice inoculated intramuscularly either with MSV-M or ascitic MSV-M tumor cells was studied. At the stages of tumor development, peak tumor size, and tumor regression the lymphoid spleen cell preparations were found to have relatively strong cytotoxic activity independent of whether the tumor was induced by MSV-M inoculation or tumor cell transplantation. However, in the latter case effector cells appeared earlier and were demonstrable for a longer period than in MSV-M-inoculated mice. Antitheta serum treatment of lymphoid spleen cells taken at the stage of peak tumor size abrogated the cytotoxic activity or diminished it considerably indicating a T-lymphocyte response.


Cytotoxic Activity Mouse Embryo Spleen Cell Inbred Mouse Embryo Cell 
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  1. 1.
    Bean, M. A., Pees, H., Fogh, J. E., Grabstald, H., Oettgen, H. F.: Cytotoxicity of lymphocytes from patients with cancer of the urinary bladder: detection by a3H-proline microcytotoxicity test. Int. J. Cancer14, 186–197 (1974)PubMedGoogle Scholar
  2. 2.
    Bean, M. A., Pees, H., Rosen, G., Oettgen, H. F.: Prelabeling target cells with3H-proline as a method for studying lymphocyte cytotoxicity. Nat. Cancer Inst. Monogr.37, 41–48 (1973)PubMedGoogle Scholar
  3. 3.
    Gorczynsky, R. M.: Immunity to murine sarcoma virus induced tumors. I. Specific thymocytes active in macrophage migration inhibition and lymphocyte transformation. J. Immunol.112, 1815–1825 (1974)PubMedGoogle Scholar
  4. 4.
    Herberman, R. B., Nunn, M. E., Lavrin, D. H.: Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. I. Distribution of reactivity and specificity. Int. J. Cancer16, 216–229 (1975)PubMedGoogle Scholar
  5. 5.
    Herberman, R. B., Nunn, M. E., Lavrin, D. H., Asofsky, R.: Effect of antibody toΘ-antigen on cell-mediated immunity induced in syngeneic mice by murine sarcoma virus. J. nat. Cancer Inst.51, 1509–1512 (1973)PubMedGoogle Scholar
  6. 6.
    Kiessling, R., Petranyi, G., Klein, G., Wigzell, H.: Genetic variation ofin vitro cytolytic activity andin vivo rejection potential of nonimmunized semi-syngeneic mice against a mouse lymphoma line. Int. J. Cancer15, 933–940 (1975)PubMedGoogle Scholar
  7. 7.
    Lamon, E. W., Skurzak, H. M., Klein, E.: The lymphocyte response to a primary viral neoplasm (MSV) through its entire course in Balb/c mice. Int. J. Cancer10, 581–588 (1972)PubMedGoogle Scholar
  8. 8.
    Lamon, E. W., Wigzell, H., Andersson, B., Klein, E.: Antitumor activityin vitro dependent on immune B lymphocytes. Nature New Biol.244, 209–211 (1973)PubMedGoogle Scholar
  9. 9.
    Lamon, E. W., Wigzell, H., Klein, E., Andersson, B., Skurzak, H. M.: The lymphocyte response to primary Moloney sarcoma virus tumors in Balb/c mice. Definition of the active subpopulations at different times after infection. J. exp. Med.137, 1472–1493 (1973)PubMedGoogle Scholar
  10. 10.
    Lavrin, D. H., Herberman, R. B., Nunn, M., Soares, N.:In vitro cytotoxicity studies of murine sarcoma virus-induced immunity in mice. J. nat. Cancer Inst.51, 1497–1508 (1973)PubMedGoogle Scholar
  11. 11.
    Plata, F., Gomard, E., Leclerc, J. C., Levy, J. P.: Further evidence for the involvement of thymus-processed lymphocytes in syngeneic tumor cell cytolysis. J. Immunol.111, 667–671 (1973)PubMedGoogle Scholar
  12. 12.
    Plata, F., Gomard, E., Leelerc, J. C., Levy, J. P.: Comparativein vitro studies on effector cell diversity in the cellular immune response to murine sarcoma virus (MSV-M)-induced tumors in mice. J. Immunol.112, 1477–1487 (1974)PubMedGoogle Scholar
  13. 13.
    Riethmüller, G., Saal, J. G., Rieber, E. P., Hinger, E., Schnellen, B., Riethmüller, D.: Cellular immune response to melanoma tumor cells. Transplant. Proc.7, 495 (1975)PubMedGoogle Scholar
  14. 14.
    Schäfer, W., Seifert, E.: Production of a potent complement fixing murine leukemia virus-antiserum from the rabbit and its reactions with various types of tissue culture cells. Virology35, 323–328 (1968)PubMedGoogle Scholar
  15. 15.
    Weiland, E., Mussgay, M.: Tumor inhibiting capacity of spleen and lymph node cells from mice with murine sarcoma virus (MSV-M)-induced tumors. Z. Immun.-Forsch.150, 414–423 (1975)Google Scholar
  16. 16.
    Weiland, E., Mussgay, M.: in preparationGoogle Scholar
  17. 17.
    Weiland, E., Weiland, F.: Transplantierbarer Aszitestumor aus einer Maus mit einem Moloneysarkom: Tumor-, Antigen- und Viruseigenschaften. Z. Immun.-Forsch.148, 151–161 (1974)Google Scholar
  18. 18.
    Winn, H. J.: Immune mechanism in homotransplantation. II Quantitative assay of the immunologic activity of lymphoid cells stimulated by tumor homografts. J. Immunol.86, 228–239 (1961)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Emilie Weiland
    • 1
  • Manfred Mussgay
    • 1
  1. 1.Federal Research Institute for Animal Virus DiseasesTübingen

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