Abstract
Different types of human papilloma viruses (HPV) had been identified by the use of bacterial restriction endonucleases (Gissmann et al., 1977). The DNAs of HPV 1, 2, and 3 showing a similar restriction enzyme pattern can be purified in sufficient quantities to permit further characterization. These viruses appear not to be identical, as far as molecular weight (4.9×106 d), buoyant density (1.700μ/ml), and sedimentation values of the different DNA components (Co I: 22–23S; Co II: 17S; Co III: 16S) are concerned.
HPV 4 DNA, which is entirely different from HPV 1–3 in its restriction enzyme pattern, does not hybridize with cRNA transcribed in vitro from HPV 1 DNA. The cRNA was shown to represent the whole viral genome.
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Gissmann, L., zur Hausen, H. Physical characterization of the deoxyribonucleic acids of different human papilloma viruses (HPV). Med Microbiol Immunol 166, 3–11 (1978). https://doi.org/10.1007/BF02121128
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DOI: https://doi.org/10.1007/BF02121128