Medical Microbiology and Immunology

, Volume 157, Issue 4, pp 335–345 | Cite as

Virusinfizierte Lymphoblasten als Antigen für die Herstellung von heterologen Antilymphocytenseren

  • G. Bandlow
  • F. Kieling
  • R. Thomssen


Nach aktiver Immunisierung mit virusinfizierten Lymphoblasten bilden Meerschweinchen vermehrt Antikörper gegen Antigene nicht infizierter Lymphocyten. Dieser immunologische Adjuvanseffekt wurde nach Infektion von menschlichen und Mäuselymphoblasten mit Herpes simplex-Virus, VSV und Vacciniavirus beobachtet. Die vermehrt gebildeten Antikörper wurden im Mikrolymphocytotoxicitätstest in vitro und in vivo durch ihre immunsuppressive Wirkung bei Tumorverpflanzung (Mastocytoma P-815-x2 DBA/2 → NMRI) nachgewiesen.

Virus infected lymphoblasts acting as antigen for the production of heterologous antilymphocyte sera


After active immunisation with virus infected lymphoblasts the production of guinea pig antibodies against antigens of not infected lymphocytes is increased. This immunological adjuvant effect was observed when human and mice lymphocytes were infected with herpes simplex virus, VSV and vacciniavirus. The increased production of antibodies was demonstrated by an in vitro micro-lymphocytotoxicitytest and in vivo by its immunsuppressive effect when allogeneic tumor cells (Mastocytoma P-815-x2 DBA/2→NMRI) were transplanted.


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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • G. Bandlow
    • 1
  • F. Kieling
    • 1
  • R. Thomssen
    • 1
  1. 1.Hygiene-Institut der Universität GöttingenDeutschland

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