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Virusinfizierte Lymphoblasten als Antigen für die Herstellung von heterologen Antilymphocytenseren

Virus infected lymphoblasts acting as antigen for the production of heterologous antilymphocyte sera

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Summary

After active immunisation with virus infected lymphoblasts the production of guinea pig antibodies against antigens of not infected lymphocytes is increased. This immunological adjuvant effect was observed when human and mice lymphocytes were infected with herpes simplex virus, VSV and vacciniavirus. The increased production of antibodies was demonstrated by an in vitro micro-lymphocytotoxicitytest and in vivo by its immunsuppressive effect when allogeneic tumor cells (Mastocytoma P-815-x2 DBA/2→NMRI) were transplanted.

Zusammenfassung

Nach aktiver Immunisierung mit virusinfizierten Lymphoblasten bilden Meerschweinchen vermehrt Antikörper gegen Antigene nicht infizierter Lymphocyten. Dieser immunologische Adjuvanseffekt wurde nach Infektion von menschlichen und Mäuselymphoblasten mit Herpes simplex-Virus, VSV und Vacciniavirus beobachtet. Die vermehrt gebildeten Antikörper wurden im Mikrolymphocytotoxicitätstest in vitro und in vivo durch ihre immunsuppressive Wirkung bei Tumorverpflanzung (Mastocytoma P-815-x2 DBA/2 → NMRI) nachgewiesen.

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Gefördert mit Hilfe von Forschungsmitteln des Landes Niedersachsen.

Die Autoren danken Frl. Angelika Weilert für ihre tatkräftige Mitarbeit.

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Bandlow, G., Kieling, F. & Thomssen, R. Virusinfizierte Lymphoblasten als Antigen für die Herstellung von heterologen Antilymphocytenseren. Med Microbiol Immunol 157, 335–345 (1972). https://doi.org/10.1007/BF02121125

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  • DOI: https://doi.org/10.1007/BF02121125

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