Abstract
347 strains from human infections were identified by gas-liquid chromatography of metabolic products and by conventional tests. Simple agar-plate assays were used to analyze the ability to form extracellular proteins. More than 90% of all strains were hemolytic on agar containing rabbit erythrocytes and all were gelatinase producers. All strains ofC. bifermentans, C. sordelli, andC. sporogenes were also caseinolytic on skimmed-milk agar, but strains ofC. perfringens, C. novyi types A and B were not. Less than 10% ofC. perfringens strains were producers of elastase and staphylolytic enzyme and all other species were non-producers. AllC. perfringens, C. novyii, C. bifermentans, andC. sordelli were lecithinase producers, butC. sporogenes was not. All strains ofC. sporogenes formed deoxyribonuclease, while a varying number of the other species showed a positive reaction.
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Nord, C.E., Wadström, T., Dornbusch, K. et al. Extracellular proteins in five clostridial species from human infections. Med Microbiol Immunol 161, 145–154 (1975). https://doi.org/10.1007/BF02121004
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DOI: https://doi.org/10.1007/BF02121004