Abstract
Concentrated murine leukemia virus (MuLV) or MuLV producing cells induce XC cell fusion within an hour leading to syncytia formation. While MuLV inactivated by UV irradiation,Β-propiolactone or hydroxylamine treatment still caused cell fusion, Bromelin- or trypsin treated MuLV was no longer able to fuse XC cells. Though sonicated MuLV induced no XC cell fusion, it interfered with cell fusion as caused by untreated MuLV. XC cells infected by diluted MuLV of a titer lower than 1×105 PFU/ml formed no syncytia although they produced MuLV. The call fusion mechanism is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bratt, M. A., Gallaher, W. R.: Preliminary analysis of the requirements for fusion from within and fusion from without by Necastle disease virus. Proc. nat. Acad. Sci. (Wash.)64, 536–543 (1969)
Chan, J. C., Vera, N., Allen, P. T., East, J. L., Knesek, J. E., Bowen, J. M., Domochowski, L.: Alteration of the syncytia-forming property of XC cells by productive Moloney leukemia virus infection. Cancer Res.35, 1854–1857 (1975)
Gelderblom, H., Bauer, H., Frank, H.: Investigations on virus production in RSV mammalian tumors. J. gen. Virol.7, 33–35 (1970)
Hartley, J. W., Rowe, W. P.: Clonal cell lines from a fetal mouse embryo which lack host range restrictions for murine leukemia viruses. Virology65, 128–134 (1975)
Hartley, J. W., Rowe, W. R., Huebner, R. J.: Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J. Virol.5, 221–225 (1970)
Hosaka, Y.: Biological activities of sonically treated Sendai virus. J. gen. Virol.8, 43–54 (1970)
Johnson, G. S., Friedmann, R. M., Pastan, I.: Analysis of the fusion of XC cells induced by homogenates of murine leukemia virus-infected cells and by purified murine leukemia virus. J. Virol.7, 753–758 (1971)
Klement, V., Nicolson, M. D., Gilden, R. V., Oroszlan, S. Sarma, P. S., Rongey, R. W., Gardner, M. B.: Rat C-type virus induced in rat sarcoma cells by 5-bromodeoxyuridine. Nature New Biol.238, 234–237 (1972)
Klement, V., Rowe, W. P., Hartley, J. W., Pugh, W. E.: Mixed culture cytopathogenicity: A new test for growth of murine leukemia virus in tissue culture. Proc. nat. Acad. Sci. (Wash.)63, 753–759 (1969)
Levitan, D. B., Blough, H. A.: Preliminary biochemical characterization of the factor(s) responsible for herpesvirus-induced exogenous fusion. J. Virol.18, 1080–1087 (1976)
Ludwig, H., Becht, H., Rott, R.: Inhibition of herpes virus-induced cell fusion by concanavalin A, antisera, and 2-deoxy-d-glucose. J. Virol.14, 307–314 (1974)
Neff, J. M., Enders, J. F.: Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus. Proc. Soc. exp. Biol. (N.Y.)127, 260–267 (1968)
Nermut, M. V., Frank, H., Schäfer, W.: Properties of mouse leukemia viruses. III. Electron microscopic appearances as revealed after conventional preparation techniques as well as freeze-drying and freeze-etching. Virology49, 345–358 (1972)
Ogura, H., Bauer, H.: Biological and electron microscopic studies on the phenotypic mixing of the thermolabile mutant of vesicular stomatitis virus, tl-17, with avian RNA tumor viruses. Arch. Virol. (in press)
Ogura, H., Friis, R.: Further evidence for the existence of a viral envelope protein defect in the Bryan high titer strain of Rous sarcoma virus. J. Virol.16, 443–446 (1975)
Rand, K., Davis, J., Gilden, R. V., Oroszlan, S., Long, C.: Fusion inhibition bioassay of a type C viral protein. Virology64, 63–74 (1975)
Rowe, W. P., Pugh, W. E., Hartley, J. W.: Plaque assay technique for murine leukemia viruses. Virology42, 1136–1139 (1970)
Schäfer, W., Rott, R.: Herstellung von Virusvaccinen mit Hydroxyamine: Verlauf der Inaktivierung und Wirkung des Hydroxyamins auf verschiedene biologische Eigenschaften einiger Viren. Z. Hyg. Infekt.-Kr.148, 256–268 (1962)
Seifert, E., Claviez, M., Frank, H., Hunsmann, G., Schwarz, H., Schäfer, W.: Properties of mouse leukemia viruses XII Produktion grö\erer Mengen von Friend-Virus durch eine permanente Zellsuspensionskultur (Eveline-Suspensions-Zellen). Z. Naturforsch.30 C, 698–700 (1975)
Svoboda, J., Chyle, P. P., Simkovic, D., Hilgert, I.: Demonstration of the absence of infectious Rous virus in rat tumuor XC whose structurally intact cells produce Rous sarcoma virus when transferred to chicks. Folia biol. (Praha)9, 77–81 (1963)
Watkins, J. F.: Cell fusion in VirologyIn: Perspectives in Virology, vol. VII (M. Pollard, ed.) pp. 159–178. Academic Press 1971
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ogura, H. XC cell fusion by murine leukemia viruses: Fusion from without. Med Microbiol Immunol 162, 175–181 (1976). https://doi.org/10.1007/BF02120995
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02120995