Abstract
Structural features of the glycosyl chains of the influenca virus have been determined. It was found that fucose was solely terminal, whereas mannose and galactose were present at the terminal as well as subterminal and core positions. Mannose and glucose molecules were shown to be branching points in the glycosyl chains. Furthermore, linkage positions of carbohydrates within the chains were characterized.
Similar content being viewed by others
References
Björndal, H., Lindberg, B., Svensson, S.: Gas-liquid chromatography of partially methylated alditols as their acetates. Acta chem. scand.21, 1801–1804 (1967)
Björndal, H., Lundblad, A.: Structure of two urinary oligosaccharides characteristic of blood group O(H)- and B-secretors. Biochim. biophys. Acta (Amst.)201, 434–437 (1970)
Chipowsky, S., Lee, Y. C., Roseman, S.: Adhesion of cultured fibroblasts to insoluble analogues of cell surface carbohydrates. Proc. nat. Acad. Sci. (Wash.)70, 2309–2312 (1973)
Chucholowius, H.-W., Rott, R.: A new method for purification of myxoviruses by zonal centrifugation with two different sucrose density gradients. Proc. Soc. exp. Biol. (N. Y.)140, 245–247 (1972)
Clamp, J. R., Bhatti, T., Chambers, R. E.: The examination of carbohydrate in glycoproteins by gas-liquid chromatography. In: Glycoproteins, edited by A. Gottschalk, pp. 300–321. Amsterdam-London-New York: Elsevier Publishing Company 1972
Etchinson, J. R., Holland, J. T.: Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus grown in four mammalian cell lines. Proc. nat. Acad. Sci. (Wash.)71, 4011–4014 (1974)
Frommhagen, L. H., Knight, C. A.: The polysaccharide and ribonucleic acid content of purified influenza virus. Virology2, 430–431 (1956)
Huang, R. T. C.: Sphingolipids of influenza viruses. Biochim. biophys. Acta (Amst.)424, 90–97 (1976)
Klenk, H.-D.: Viral envelopes and their relationship to cellular membranes. Curr. Top. Microbiol. Immunol.68, 29–58 (1974)
Laine, R., Söderland, H., Renkonen, O.: Chemical composition of Semliki Forest virus. Intervirology1, 110–118 (1973)
Sweeley, C. C., Bentley, R., Makita, M., Wells, W. W.: Gas-liquid chromatography of trimethyl silyl derivatives of sugars and related substances. J. Amer. chem. Soc.85, 2417–2507 (1963)
Author information
Authors and Affiliations
Additional information
This work was supported by the Sonderforschungsbereich 47 (Virologie). The constructive criticism and valuable suggestions of R. Rott and H.-D. Klenk are very much appreciated.
Rights and permissions
About this article
Cite this article
Huang, R.T.C. On the structure of the carbohydrate chains of different strains of the influenza virus. Med Microbiol Immunol 162, 169–173 (1976). https://doi.org/10.1007/BF02120994
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02120994