Medical Microbiology and Immunology

, Volume 158, Issue 3, pp 209–217 | Cite as

Activation of herpesvirus from normal bovine fetal spleen cells after prolonged cultivation

  • H. Ludwig
  • J. Storz


Five identical viral isolates were recovered from bovine fetal spleen cells maintained in culture for 30 to 67 days. During this time the cells were sub-cultured 3 to 8 times. Cells cultured from other organs of the 3 donor fetuses did not evidence lytic viral infections. The viral isolates were identified serologically, morphologically, culturally and by characterization of the virus DNA as the herpesvirus of infectious bovine rhinotracheitis. The available data indicate latent infection of the donor fetuses as the source of these viral isolates.


Viral Infection Spleen Cell Viral Isolate Latent Infection Prolonged Cultivation 
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5 identische Virusisolate wurden aus fetalen bovinen Milzzellen gewonnen, die 30–67 Tage in Kultur gehalten worden waren. Während dieser Zeit wurden die Zellen 3–8 mal subkultiviert. Die Zellkulturen anderer Organe von 3 Spenderfeten zeigten keine Hinweise einer lytischen Virusinfection. Die Virusisolate wurden morphologisch, biologisch und durch Charakterisierung ihrer Virus-DNS als Herpesvirus der Infektiösen Bovinen Rhinotracheitis identifiziert. Die erfaßbaren Daten deuten darauf hin, daß das Virus in latenter Form in den Spenderfeten vorhanden war.


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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • H. Ludwig
    • 1
  • J. Storz
    • 1
  1. 1.Institut für VirologieJustus Liebig-UniversitätGiessen

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