Medical Microbiology and Immunology

, Volume 158, Issue 3, pp 209–217 | Cite as

Activation of herpesvirus from normal bovine fetal spleen cells after prolonged cultivation

  • H. Ludwig
  • J. Storz


Five identical viral isolates were recovered from bovine fetal spleen cells maintained in culture for 30 to 67 days. During this time the cells were sub-cultured 3 to 8 times. Cells cultured from other organs of the 3 donor fetuses did not evidence lytic viral infections. The viral isolates were identified serologically, morphologically, culturally and by characterization of the virus DNA as the herpesvirus of infectious bovine rhinotracheitis. The available data indicate latent infection of the donor fetuses as the source of these viral isolates.


Viral Infection Spleen Cell Viral Isolate Latent Infection Prolonged Cultivation 


5 identische Virusisolate wurden aus fetalen bovinen Milzzellen gewonnen, die 30–67 Tage in Kultur gehalten worden waren. Während dieser Zeit wurden die Zellen 3–8 mal subkultiviert. Die Zellkulturen anderer Organe von 3 Spenderfeten zeigten keine Hinweise einer lytischen Virusinfection. Die Virusisolate wurden morphologisch, biologisch und durch Charakterisierung ihrer Virus-DNS als Herpesvirus der Infektiösen Bovinen Rhinotracheitis identifiziert. Die erfaßbaren Daten deuten darauf hin, daß das Virus in latenter Form in den Spenderfeten vorhanden war.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bartha, A., Juhasz, M., Liebermann, H.: Isolation of a bovine herpesvirus from calves with respiratory disease and kerato-conjunctivitis. Acta vet. Acad. Sci. hung.16, 357–358 (1966).PubMedGoogle Scholar
  2. Benyesh-Melnick, M., Rosenberg, H. S., Watson, B.: Viruses in cell cultures of kidneys of children with congenital heart malformations and other diseases. Proc. Soc. exp. Biol. (N. Y.)117, 452–459 (1964).Google Scholar
  3. Black, P. H., Hartley, K. W., Rowe, W. P.: Isolation of a cytomegalovirus from African green monkey. Proc. Soc. exp. Biol. (N. Y.)112, 601–605 (1963).Google Scholar
  4. Bowling, C. P., Goodheart, C. R., Plummer, G.: Oral and genital bovine herpesviruses. J. Virol.3, 95–97 (1969).PubMedGoogle Scholar
  5. Darlington, R. W., Moss, L. H.: The envelope of herpesvirus. Progr. med. Virol.11, 16–45 (1969).Google Scholar
  6. Falk, L. A., Wolfe, L. G., Hoekstra, J., Deinhardt, F.: Demonstration of herpesvirus saimiri-associated antigens in peripheral lymphocytes from infected marmosets during in vitro cultivation. J. nat. Cancer Inst.48, 523–530 (1972).PubMedGoogle Scholar
  7. Graham, B. J., Ludwig, H., Bronson, D. L., Benyesh-Melnick, M., Biswal, N.: Physicochemical properties of the DNA of herpes viruses. Biochim. biophys. Acta (Amst.)259, 13–23 (1972).Google Scholar
  8. Hampar, B., Derge, J. G., Martos, L. M., Walker, J. L.: Synthesis of Epstein-Barr Virus after activation of the viral genome in a “virus negative” human lymphoblastoid cell (Raji) made resistant to 5-Bromodeoxyuridine. Proc. nat. Acad. Sci. (Wash.)69, 78–82 (1972).Google Scholar
  9. Henson, D., Strano, A. J., Slotnik, M., Goodheart, C.: Mouse cytomegalovirus: Isolation from spleen and lymph nodes of chronically infected mice. Proc. Soc. exp. Biol. (N. Y.)140, 802–806 (1972).Google Scholar
  10. Hsiung, G. D.: Latent virus infections in primate tissues with special reference to Simian viruses. Bact. Rev.32, 185–205 (1968).PubMedGoogle Scholar
  11. Hsiung, G. D., Fischman, H. R., Fong, C. K. Y., Green, R. H.: Characterization of a cytomegalo-like virus isolated from spontaneously degenerated equine kidney cell culture. Proc. Soc. exp. Biol. (N. Y.)130, 81–84 (1969).Google Scholar
  12. Kendrick, J. W., Sträub, O. C.: Infectious bovine rhinotracheitis—infectious pustular vulvovaginitis virus infection in pregnant cows. Amer. J. vet. Res.28, 1269–1282 (1967).PubMedGoogle Scholar
  13. Ludwig, H., Paulsen, J., Kaminjolo, J. S.: Kälbercornea-Zellen in Kultur. I. Eigenschaften einer fibroblasten- und einer epithelartigen Zellinie und deren Eignung für die Untersuchung einiger tierpathogener Viren. Z. med. Mikrobiol. u. Immunol.155, 133–149 (1969).Google Scholar
  14. Ludwig, H., Biswal, N., Bryans, J. T., McCombs, R.: Some properties of the DNA from a new equine herpesvirus. Virology45, 534–537 (1971).CrossRefPubMedGoogle Scholar
  15. Luther, P. D., Bradley, P. G., Haig, D. A.: The isolation and characterization of a herpesvirus from calf kidney cell cultures. Res. Vet. Sci.12, 496–498 (1971).PubMedGoogle Scholar
  16. Madin, S. H., York, C. J., McKercher, D. G.: Isolation of the infectious bovine rhinotracheitis virus. Science124, 721–722 (1956).PubMedGoogle Scholar
  17. Malmquist, W. A., Van Der Maaten, M. J., Boothe, A. D.: Isolation, immuno-diffusion, immunofluorescence, and electron microscopy of a syncitial virus of lymphosarcomatous and apparently normal cattle. Cancer Res.29, 188–200 (1969).PubMedGoogle Scholar
  18. Martin, W. B., Martin, B., Hay, D., Lauder, I. M.: Bovine ulcerative mammillitis caused by a herpesvirus. Vet. Rec.78, 494–497 (1966).PubMedGoogle Scholar
  19. McKercher, G. D., Wada, E. M.: The virus of infectious bovine rhinotracheitis as a cause of abortion in cattle. J. Amer. vet. med. Ass.144, 136–142 (1964).PubMedGoogle Scholar
  20. Mohanty, S. B., Hammond, R. C., Lillie, M. G.: A new bovine herpesvirus and its effect on experimentally infected calves. Arch. ges. Virusforsch.34, 394–395 (1971).CrossRefGoogle Scholar
  21. Nesburn, A. B.: Isolation and characterization of a herpes-like virus from New Zealand albino rabbit kidney cell cultures. J. Virol.3, 59–69 (1969).PubMedGoogle Scholar
  22. Plowright, W., Ferris, R. D., Scott, G. R.: Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever. Nature (Lond.)188, 1167–1169 (1960).Google Scholar
  23. Russel, W. C., Crawford, L. C.: Properties of the nucleic acids from some herpes group viruses. Virology22, 288–292 (1964).CrossRefPubMedGoogle Scholar
  24. Sheffy, B. E., Davies, H.: Reactivation of a bovine herpesvirus after corticosteroid treatment. Proc. Soc. exp. Biol. (N. Y.)140, 974–976 (1972).Google Scholar
  25. Sterz, H., Ludwig, H.: Plaque test and biological properties of bovine herpes mammilitis (BHM) virus. Zbl. Vet.-Med. B 473–478 (1972).Google Scholar
  26. Storz, J.: Comments on malignant catarrhal fever. J. Amer. vet. med. Ass.152, 804–806 (1968).Google Scholar
  27. Swack, N. S., Hsiung, G. D.: Studies of a rabbit herpes-like virus. Proc. Soc. exp. Biol. (N.Y.)140, 1330–1333 (1972).Google Scholar
  28. Van Der Maaten, M. J., Boothe, A. D.: Isolation of a herpes-like virus from lymphosarcomatous cattle. Arch. ges. Virusforsch.37, 85–96 (1972).CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • H. Ludwig
    • 1
  • J. Storz
    • 1
  1. 1.Institut für VirologieJustus Liebig-UniversitätGiessen

Personalised recommendations