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Malignant lymphoma induced by partially purifiedHerpesvirus saimiri and recovery of infectious virus from tumorous lymph nodes

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Summary

Supernatant of OMK cells infected withHerpesvirus saimiri (HVS) and partially purified HVS were shown to induce malignant lymphoma in New World monkeys (Saguinus oedipus). Radioactively labeled HVS was partially purified by velocity sedimentation in sucrose and buoyant density centrifugation in potassium tartrate and inoculated in a monkey. Infectious HVS was isolated from a tumorous lymph node of this animal 2 h after its cultivationin vitro. In contrast to the lymphocytes from fresh peripheral blood cocultivation of the cells obtained from the lymph node was not necessary for the isolation of the virus. The development of comparatively high amounts of neutralizing antibodies against HVS in the sera of the fatally diseased monkeys is an indication for a replication of HVS in the tumor bearing animals.

Zusammenfassung

Der Gewebekulturüberstand von mitHerpesvirus saimiri (HVS) infizierten OMK-Zellen und partiell gereinigtes HVS induzierten in Marmoset Affen (Saguinus oedipus) die Entwicklung von malignen Lymphomen. Radioaktiv markiertes HVS wurde durch Sedimentation im Saccharosegradienten und anschließende Dichtezentrifugation im Kaliumtartratgradienten partiell gereinigt und in einen Affen inoculiert. Bei diesem Tier konnte infektiöses HVS von einem tumorösen Lymphknoten 2 Std nach dessen Kultivationin vitro isoliert werden. Im Gegensatz zu den Lymphocyten aus frischem peripheren Blut war eine Kokultivation der Zellen aus dem Lymphknoten für die Isolierung des Virus nicht erforderlich. Die Entwicklung vergleichsweise hoher Titer neutralisierender Antikörper in den Seren der an einem Tumor erkrankten Tiere kann als Hinweis dafür gewertet werden, daß sich das HVS in diesen Affenin vivo vermehrt.

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Authors and Affiliations

  1. Hygiene-Institut der Universität, Göttingen, Germany

    Rainer Laufs & Bernhard Fleckenstein

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  1. Rainer Laufs
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  2. Bernhard Fleckenstein
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Laufs, R., Fleckenstein, B. Malignant lymphoma induced by partially purifiedHerpesvirus saimiri and recovery of infectious virus from tumorous lymph nodes. Med Microbiol Immunol 158, 135–146 (1972). https://doi.org/10.1007/BF02120479

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  • DOI: https://doi.org/10.1007/BF02120479

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