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Purification of influenza virus hemagglutinin by affinity chromatography

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Summary

Influenza virus or viral hemagglutinin could be purified on columns of agarose to which receptor substance from erythrocytes or fetuin had been conjugated. Hemagglutinin was eluted by elevating the salt concentration and temperature, while neuraminidase was kept inactive. These conditions permitted repeated use of the column.

Zusammenfassung

Influenzavirus oder virales Hämagglutinin konnte auf Agarose-Säulen gereinigt werden, an die Receptorsubstanz aus Erythrocyten oder Fetuin konjugiert worden war. Das Hämagglutinin wurde durch Erhöhung der Salzkonzentration und der Temperatur eluiert, während die Neuraminidase inaktiv gehalten wurde. Auf diese Weise ließen sich die Säulen wiederholt verwenden.

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Authors and Affiliations

  1. Institut für Virologie, Justus Liebig-Universität, Gießen, Germany

    H. Becht & R. Rott

Authors
  1. H. Becht
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  2. R. Rott
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Additional information

The technical assistance of Mrs. H. Pahlich is gratefully acknowledged. The work was supported by the Sonderforschungsbereich 47 (Virologie).

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Becht, H., Rott, R. Purification of influenza virus hemagglutinin by affinity chromatography. Med Microbiol Immunol 158, 67–70 (1972). https://doi.org/10.1007/BF02120470

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  • DOI: https://doi.org/10.1007/BF02120470

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