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The detection of A and B antigens on human hair by the absorption-elution technique using LISS and papain-treated test cells

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Summary

The absorption-elution technique with low ionic strength solution (LISS) and papain-treated test cells previously used for bloodstains was employed for the detection of AB0 antigens on human hair. Antigen identification was always possible, with good intensity of agglutination, even in those cases where classic techniques had given false-negative results. It was possible to obtain positive results with fragments of human hair as small as 0.2 cm.

Zusammenfassung

Die schon für Blutproben verwendete Absorbtions-Elutionsmethode mit LISS (Lösung mit schwach wirkender Ionenstärke) und Testerythrozytensensibilisierung mit Papain wurde von den Verfassern zur Identifizierung der AB0-Antigene in menschlichen Haaren angewandt. Die Identifizierung der Antigene gelang immer mit einer guten Agglutinations-intensität auch in solchen Fällen, in denen die herkömmliche Methodik zu einer falschen Negativität geführt hatte. Die Mindestlänge der Haare für die positive Reaktion war 0,2 cm.

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Cortivo, P., Biasiolo, M., Scorretti, C. et al. The detection of A and B antigens on human hair by the absorption-elution technique using LISS and papain-treated test cells. Z Rechtsmed 91, 195–199 (1984). https://doi.org/10.1007/BF02116422

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