Immunogenetics

, Volume 31, Issue 5–6, pp 361–367 | Cite as

Post-transcriptional regulation of complement C4 in low C4-producing strain of mouse

  • Kohzo Nakayama
  • Sa-nga Pattanakitsakul
  • Shigeru Yokoyama
  • Masaru Nonaka
  • Morinobu Takahashi
  • Hiroshi Kimura
Article

Abstract

The expression of the fourth component of complement (C4) of the mouse can differ 20-fold and is determined byC4-high (C4 h ) orC4-low (C4 l ) alleles. To investigate the molecular mechanisms underlying the differences in C4 expression, we compared the transcriptional activity of theC4 genes between high and low C4-producer strains of mice (B10 and FM vs B10.BR) using nuclear transcriptional and chloramphenicol acetyltransferase (CAT) assays. We also compared the level of C4-specific RNA in total and nuclear RNA of the liver. The results revealed no significant difference in transcriptional activity betweenC4 h andC4 l genes. However, the steady-state levels of C4 mRNA are ten times lower inC4 l strains than inC4 h strains, suggesting that the major regulation of C4 plasma levels occurs at the post-transcriptional level.

Keywords

Molecular Mechanism Plasma Level Transcriptional Activity Chloramphenicol Major Regulation 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Kohzo Nakayama
    • 1
  • Sa-nga Pattanakitsakul
    • 1
  • Shigeru Yokoyama
    • 1
  • Masaru Nonaka
    • 1
  • Morinobu Takahashi
    • 1
  • Hiroshi Kimura
    • 2
  1. 1.Department of Immunobiology, Cancer Research InstituteKanazawa UniversityKanazawaJapan
  2. 2.Department of Experimental RadiologyShiga University of Medical ScienceOtsu, ShigaJapan

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