Zeitschrift für Rechtsmedizin

, Volume 74, Issue 4, pp 273–281 | Cite as

Histamine and serotonin in the gastric erosions of rats dead from exposure to cold: A histochemical and quantitative study

  • J. Hirvonen
  • R. Elfving
Originalarbeiten — Original Papers

Summary

Rats were kept at −20°C until they died. Stomach erosions were found in every rat exposed to cold. Histamine and serotonin were visualized by fluorescence microscopy and their quantities were measured fluorometrically.

Histamine fluorescence had disappeared in the erosion region from mucosal mast cells and enterochromaffin-like cells. Submucosal mast cells had retained full fluorescence and showed no degranulation. The amine content showed an insignificant decrease. Serotonin fluorescence had also disappeared from the mucosal mast cells in the erosion, but was visible in enterochromaffin cells and submucosal mast cells beneath the erosion. Degranulation of mucosal mast cells was confirmed in sections stained by toluidine blue. Gastric serotonin level was significantly lower in the test group than in the control group (0.58±0.23 μ/g versus 0.99±0.28 μ/g). The results confirmed the effectiveness of cold exposure in causing gastric erosion in the rat. Direct evidence of the release of histamine and serotonin from the mucosal cells during formation of erosions was obtained.

Key words

Hypothermia death, gastric erosions Gastric erosions, hypothermia death 

Zusammenfassung

Ratten wurden bei −20°C gehalten bis sie starben. Magenerosionen wurden in jeder der Kälte ausgesetzten Ratte gefunden. Histamin und Serotonin wurden sichtbar gemacht mittels der Fluorescenzmikroskopie, und ihre Mengen wurden fluorometrisch gemessen.

Histamine-Fluorescenz war in der zersetzten Zone der Schleimhautmastzellen und enterochromaffinähnlichen Zellen verschwunden. Untere Mastzellen enthielten volle Fluorescenz und zeigten keine Degranulation. Der Amingehalt zeigte einen unbedeutenden Abfall. Serotonin-Fluorescenz war gleichfalls aus den Schleimhautmastzellen verschwunden, war aber sichtbar in Enterochromaffinzellen unterhalb der Zersetzung. Degranulation der Schleimhautmastzellen wurde bestätigt in Schnitten, die mit Toluidinblau gefärbt waren. Der gastrische Serotoningehalt war bemerkenswert niedriger in der Testgruppe als in der Kontrollgruppe (0,58 ± 0,23 μ/g versus 0,99 ± 0,28 μ/g). Die Resultate bestätigten die Effektivität der Kälteeinwirkung, um gastrische Erosionen der Ratte zu verursachen. Direkter Beweis für die Freisetzung von Histamin und Serotonin aus den Schleimhautzellen während der Bildung von Erosionen wurde erreicht.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • J. Hirvonen
    • 1
  • R. Elfving
    • 1
  1. 1.Department of Forensic MedicineUniversity of OuluOulu 23Finland

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