Abstract
The effects of vitamin A, selenium and butylated hydroxytoluene (BHT) on the growth of a human tongue cancer cell line were examined in monolayer cell cultures. A colony-forming assay showed a 50% reduction in the survival rate of the cell line at a concentration of 2.6 μg/ml selenium, 60 I μg/ml vitamin A and/or 38 μg/ml BHT. Relatively low concentrations of selenium markedly inhibited glucose consumption. Flow cytometric analysis with both fluorescein-isothiocyanate-labelled bromodeoxyuridine monoclonal antibody and propidium iodide demonstrated an increase in S-phase fractions 1 day after the addition of selenium, increased GO/G1 phase fractions in the presence of vitamin A and increased G2M phase fractions when BHT was added. These results suggest that these compounds inhibit DNA synthesis of in vitro human tongue cancer cells by different mechanisms.
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Inoue, I., Yamamoto, Y., Takasaki, T. et al. Inhibitory effects of selenium, vitamin A and butylated hydroxytoluene on in vitro growth of human tongue cancer cells. Eur Arch Otorhinolaryngol 252, 509–512 (1995). https://doi.org/10.1007/BF02114762
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DOI: https://doi.org/10.1007/BF02114762