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Archives of gynecology

, Volume 233, Issue 3, pp 155–164 | Cite as

In vitro inhibition of rat oocyte meiosis by human follicular fluid fractions

  • S. Chari
  • T. Hillensjö
  • C. Magnusson
  • G. Sturm
  • E. Daume
Original Papers

Summary

We studied the effect of low molecular weight fractions of human follicular fluid (FF1) on rat oocyte meiosis and progesterone secretion by the granulosa cells. Steroid-free FF1 extracts were filtered through Sephadex G50 gel. One highly retarded fraction was obtained (GF-3), which was either used for testing or further purified by filtration on Sephadex G10, the G10-3 fraction of which was used for experiments.

The GF-3 and G10-3 fractions of FF1 inhibited (in a dose-dependent and reversible manner) meiosis of isolated rat oocytes during a 4-h culture. Similarly treated fractions of serum had no effect. The inhibition was not abolished by ether extraction, trypsin treatment, heating to 56° C for 1 h or boiling for 5 min, whereas heating to 105° C for 18 h decreased the effect.

Gonadotropin-stimulated progesterone secretion by cumulus and mural granulosa cells was also dose-dependently and reversibly inhibited by the GF-3 fractions. Our results confirm those obtained in earlier studies on porcine oocyte maturation inhibitor (OMI).

Key words

Follicular fluid Oocyte Meiosis Progesterone 

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References

  1. Charming CP (1979) Follicular non-steroidal regulators. In: Channing CP, Marsh JM, Sadler WA (eds) Ovarian follicular and corpus luteum function. Plenum Press, New York, pp 327–345Google Scholar
  2. Channing CP, Anderson LD, Hoover DJ, Kolena J, Osteen KG, Pomerantz SH, Tanabe K (1982) The role of non-steroidal regulators in control of oocyte and follicular maturation. Rec Prog Horm Res 38: 331–400PubMedGoogle Scholar
  3. Chari S, Hopkinson CRN, Daume E, Sturm G (1979) Purification of “inhibin” from human ovarian follicular fluid. Acta Endocrinol (Copenh) 90: 157–166Google Scholar
  4. Chari S, Aumüller G, Daume E, Sturm G, Hopkinson C (1981) The effects of human follicular fluid inhibin on the morphology of the ovary of the immature rat. Arch Gynecol 230: 239–249PubMedGoogle Scholar
  5. Daume E, Chari S, Sturm G, Hirchhauser C (1977) Existence of inhibin activity in human follicular fluid. Acta Endocrinol [Suppl 212] (Copenh) 85: 56Google Scholar
  6. Daume E, Chari S, Sturm G, Hillensjö T, Magnusson C (1981) Nonsteroidal ovarian inhibitors in human follicular fluid. In: Rolland R, van Hall EV, Hillier SG, McNatty KP, Schoemaker J (eds) Follicular maturation and ovulation. Excerpta Medica, Amsterdam Oxford Princeton, pp 237–248Google Scholar
  7. Hillensjö T, Batta SK, Kripner AS, Wentz AC, Sulewski J, Channing CP (1978) Inhibitory effect of human follicular fluid upon the maturation of porcine oocytes in culture. J Clin Endocrinol Metab 47: 1332–1335PubMedGoogle Scholar
  8. Hillensjö T, Kripner AS, Pomerantz SH, Channing CP (1979) Action of porcine follicular fluid oocyte maturation inhibitor in vitro: Possible role of the cumulus cells. In: Channing CP, Marsh JM, Sadler WA (eds) Ovarian follicular and corpus luteum function. Plenum Press, New York, pp 283–291Google Scholar
  9. Hillensjö T, Pomerantz SH, Kripner AS, Anderson LD, Channing CP (1980) Inhibition of cumulus cell progesterone secretion by low molecular weight fractions of porcine follicular fluid which also inhibit oocyte maturation. Endocrinology 106: 584–591PubMedGoogle Scholar
  10. Hillensjö T, Chari S, Magnusson C, Daume E, Sturm G (1981a) Inhibitory effects of low molecular weight fractions of human follicular fluid upon granulosa cells and oocytes in vitro. In: Semm K, Mettler L (eds) Human reproduction. Excerpta Medica, Amsterdam Oxford Princeton, pp 458–462Google Scholar
  11. Hillensjö T, Magnusson C, Svensson U, Thelander H (1981b) Effect of LH and FSH on progesterone synthesis in cultured rat cumulus cells. Endocrinology 108: 1920–1924PubMedGoogle Scholar
  12. Hopkinson CRN, Sturm G, Daume E, Fritze E, Hirchhauser C (1975) Nonsteroidal inhibition of FSH secretion by bovine follicular fluid in male and female rats. Int Res Comm Sys 3: 588Google Scholar
  13. Jong FH De, Sharpe RM (1976) Evidence for inhibin-like activity in bovine follicular fluid. Nature 263: 71–72CrossRefPubMedGoogle Scholar
  14. Ledwitz-Rigby F, Rigby BW, Gay VL, Stetson M, Young J, Channing CP (1977) Inhibitory action of porcine follicular fluid upon granulosa cell luteinization in vitro: assay and influence of follicular maturation. J Endocrinol 74: 175–184PubMedGoogle Scholar
  15. Magnusson C, Hillensjö T, Tsafriri A, Hultborn R, Ahrén K (1977) Oxygen consumption of maturing rat oocytes. Biol Reprod 17: 9–15PubMedGoogle Scholar
  16. Pomerantz SH, Channing CP, Tsafriri (1979) Studies on the purification and action of an oocyte maturation inhibitor isolated from porcine follicular fluid. In: Gross E, Meienhofer J (eds) Peptides: Structure and biological function. Pierce Chemical Co., pp 765–774Google Scholar
  17. Stone SL, Pomerantz SH, Schwartz-Kripner A, Channing CP (1978) Inhibitor of oocyte maturation from porcine follicular fluid: Further purification and evidence for reversible action. Biol Reprod 19: 585–592PubMedGoogle Scholar
  18. Tennant JR (1964) Evaluation of the trypan blue technique for determination of cell viability. Transplantation 2: 685–694Google Scholar
  19. Tsafriri A, Channing CP (1975) An inhibitory influence of granulosa cells and follicular fluid upon porcine oocyte meiosis in vitro. Endocrinology 96: 922–927PubMedGoogle Scholar
  20. Tsafriri A, Pomerantz SH, Channing CP (1976) Inhibition of oocyte maturation by porcine follicular fluid: Partial characterisation of the inhibitor. Biol Reprod 14: 511–516PubMedGoogle Scholar
  21. Tsafriri A, Channing CP, Pomerantz SH, Lindner HR (1977) Inhibition of maturation of isolated rat oocytes by porcine follicular fluid. J Endocrinol 75: 285–291PubMedGoogle Scholar
  22. Tsafriri A, Dekel N, Bar Ami S (1982) The role of oocyte maturation inhibitor in follicular regulation of oocyte maturation. J Reprod Fertil 64: 541–551PubMedGoogle Scholar
  23. Woolf CM (1968) Principles of biometry, 1st edn. van Nostrand Co., Princeton, New JerseyGoogle Scholar

Copyright information

© J. F. Bergmann Verlag 1983

Authors and Affiliations

  • S. Chari
    • 1
  • T. Hillensjö
    • 2
  • C. Magnusson
    • 2
  • G. Sturm
    • 1
  • E. Daume
    • 1
  1. 1.Dept. of Obstetrics and GynecologyUniversity of MarburgMarburg/LahnFederal Republic of Germany
  2. 2.Dept. of PhysiologyUniversity of GöteborgGöteborgSweden

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