Archives of gynecology

, Volume 233, Issue 3, pp 155–164 | Cite as

In vitro inhibition of rat oocyte meiosis by human follicular fluid fractions

  • S. Chari
  • T. Hillensjö
  • C. Magnusson
  • G. Sturm
  • E. Daume
Original Papers


We studied the effect of low molecular weight fractions of human follicular fluid (FF1) on rat oocyte meiosis and progesterone secretion by the granulosa cells. Steroid-free FF1 extracts were filtered through Sephadex G50 gel. One highly retarded fraction was obtained (GF-3), which was either used for testing or further purified by filtration on Sephadex G10, the G10-3 fraction of which was used for experiments.

The GF-3 and G10-3 fractions of FF1 inhibited (in a dose-dependent and reversible manner) meiosis of isolated rat oocytes during a 4-h culture. Similarly treated fractions of serum had no effect. The inhibition was not abolished by ether extraction, trypsin treatment, heating to 56° C for 1 h or boiling for 5 min, whereas heating to 105° C for 18 h decreased the effect.

Gonadotropin-stimulated progesterone secretion by cumulus and mural granulosa cells was also dose-dependently and reversibly inhibited by the GF-3 fractions. Our results confirm those obtained in earlier studies on porcine oocyte maturation inhibitor (OMI).

Key words

Follicular fluid Oocyte Meiosis Progesterone 


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Copyright information

© J. F. Bergmann Verlag 1983

Authors and Affiliations

  • S. Chari
    • 1
  • T. Hillensjö
    • 2
  • C. Magnusson
    • 2
  • G. Sturm
    • 1
  • E. Daume
    • 1
  1. 1.Dept. of Obstetrics and GynecologyUniversity of MarburgMarburg/LahnFederal Republic of Germany
  2. 2.Dept. of PhysiologyUniversity of GöteborgGöteborgSweden

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