In vitro inhibition of rat oocyte meiosis by human follicular fluid fractions
We studied the effect of low molecular weight fractions of human follicular fluid (FF1) on rat oocyte meiosis and progesterone secretion by the granulosa cells. Steroid-free FF1 extracts were filtered through Sephadex G50 gel. One highly retarded fraction was obtained (GF-3), which was either used for testing or further purified by filtration on Sephadex G10, the G10-3 fraction of which was used for experiments.
The GF-3 and G10-3 fractions of FF1 inhibited (in a dose-dependent and reversible manner) meiosis of isolated rat oocytes during a 4-h culture. Similarly treated fractions of serum had no effect. The inhibition was not abolished by ether extraction, trypsin treatment, heating to 56° C for 1 h or boiling for 5 min, whereas heating to 105° C for 18 h decreased the effect.
Gonadotropin-stimulated progesterone secretion by cumulus and mural granulosa cells was also dose-dependently and reversibly inhibited by the GF-3 fractions. Our results confirm those obtained in earlier studies on porcine oocyte maturation inhibitor (OMI).
Key wordsFollicular fluid Oocyte Meiosis Progesterone
Unable to display preview. Download preview PDF.
- Charming CP (1979) Follicular non-steroidal regulators. In: Channing CP, Marsh JM, Sadler WA (eds) Ovarian follicular and corpus luteum function. Plenum Press, New York, pp 327–345Google Scholar
- Chari S, Hopkinson CRN, Daume E, Sturm G (1979) Purification of “inhibin” from human ovarian follicular fluid. Acta Endocrinol (Copenh) 90: 157–166Google Scholar
- Daume E, Chari S, Sturm G, Hirchhauser C (1977) Existence of inhibin activity in human follicular fluid. Acta Endocrinol [Suppl 212] (Copenh) 85: 56Google Scholar
- Daume E, Chari S, Sturm G, Hillensjö T, Magnusson C (1981) Nonsteroidal ovarian inhibitors in human follicular fluid. In: Rolland R, van Hall EV, Hillier SG, McNatty KP, Schoemaker J (eds) Follicular maturation and ovulation. Excerpta Medica, Amsterdam Oxford Princeton, pp 237–248Google Scholar
- Hillensjö T, Kripner AS, Pomerantz SH, Channing CP (1979) Action of porcine follicular fluid oocyte maturation inhibitor in vitro: Possible role of the cumulus cells. In: Channing CP, Marsh JM, Sadler WA (eds) Ovarian follicular and corpus luteum function. Plenum Press, New York, pp 283–291Google Scholar
- Hillensjö T, Chari S, Magnusson C, Daume E, Sturm G (1981a) Inhibitory effects of low molecular weight fractions of human follicular fluid upon granulosa cells and oocytes in vitro. In: Semm K, Mettler L (eds) Human reproduction. Excerpta Medica, Amsterdam Oxford Princeton, pp 458–462Google Scholar
- Hopkinson CRN, Sturm G, Daume E, Fritze E, Hirchhauser C (1975) Nonsteroidal inhibition of FSH secretion by bovine follicular fluid in male and female rats. Int Res Comm Sys 3: 588Google Scholar
- Pomerantz SH, Channing CP, Tsafriri (1979) Studies on the purification and action of an oocyte maturation inhibitor isolated from porcine follicular fluid. In: Gross E, Meienhofer J (eds) Peptides: Structure and biological function. Pierce Chemical Co., pp 765–774Google Scholar
- Tennant JR (1964) Evaluation of the trypan blue technique for determination of cell viability. Transplantation 2: 685–694Google Scholar
- Woolf CM (1968) Principles of biometry, 1st edn. van Nostrand Co., Princeton, New JerseyGoogle Scholar