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Acta Biotheoretica

, Volume 25, Issue 1, pp 44–65 | Cite as

Positional information in the amphibian limb

  • J. Faber
Article

Summary

(1) The concept of positional information is applied to a large amount of data obtained previously in experiments on developing and regenerating amphibian limbs. Only the proximo-distal axis of the limb is considered. It is shown that the concept provides a simple, unitary hypothesis which satisfactorily accounts for the experimental data, and may moreover suggest meaningful new approaches. (2) It is suggested that the boundaries of the bipolar limb system lie in the girdle skeleton and at the distal end of the limb, respectively, and that it is the apical epidermis of the growing or regenerating limb which defines the distal boundary conditions. A relatively stable gradient of positional information is assumed to be set up in the mesoderm (or mesenchyme). It is further shown that the differentiated limb retains its positional information and upon amputation imparts it to the base of the blastema. (3) To explain an apparent discrepancy between the developing and the regenerating limb, it is proposed that dedifferentiation of mesodermal limb tissues upon amputation entails a change of positional value in the mesenchyme. Consequently, the amputation level does not once and for all specify the positional value at the proximal end of a mass of blastemal mesenchyme, particularly when the mesenchyme is transplanted in such a way that its linear size decreases (regression) or increases (fusion of several blastemas).

Keywords

Boundary Condition Apparent Discrepancy Limb System Linear Size Positional Information 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Leiden University Press 1976

Authors and Affiliations

  • J. Faber
    • 1
  1. 1.Hubrecht LaboratoryUtrechtNetherlands

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