Fish Physiology and Biochemistry

, Volume 15, Issue 4, pp 333–338 | Cite as

Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos

  • Kozo Inaba
  • Masaharu Seno
  • Hisaya Morino
Article
  • 44 Downloads

Abstract

Heparin-binding fractions were taken from the heparin sepharose columns on which extracts of loach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4–8 and 12–16 somites stages were applied. These heparin-binding fractions, except the fraction derived from 12–16 somite embryos, showed potent mitogenic activity on fibroblast-like cells derived from caudal fin blastema of goldfish. Western blot analysis of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors (FGF-1 and-2). An immunoreactive FGF-1 band at 16 kD was detected in the heparin-binding fraction derived from embryos in each stage of blastula, gastrula and 4–8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4–8 somite embryos. In the heparin-binding fraction derived from 12–16 somite embryos neither immunoreactive FGF-1 nor-2 member band was detectable.

Keywords

fish FGF-1 fish FGF-2 fish embryos cell proliferation heparin affinity Western blot 

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Copyright information

© Kugler Publication bv Amsterdam 1996

Authors and Affiliations

  • Kozo Inaba
    • 1
  • Masaharu Seno
    • 2
  • Hisaya Morino
    • 1
  1. 1.Biological InstituteHyogo University of Teachers EducationHyogoJapan
  2. 2.Department of Bioengineering Science, Faculty of EngineeringOkayama UniversityTsushima, OkayamaJapan

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