Fish Physiology and Biochemistry

, Volume 15, Issue 4, pp 333–338 | Cite as

Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos

  • Kozo Inaba
  • Masaharu Seno
  • Hisaya Morino


Heparin-binding fractions were taken from the heparin sepharose columns on which extracts of loach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4–8 and 12–16 somites stages were applied. These heparin-binding fractions, except the fraction derived from 12–16 somite embryos, showed potent mitogenic activity on fibroblast-like cells derived from caudal fin blastema of goldfish. Western blot analysis of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors (FGF-1 and-2). An immunoreactive FGF-1 band at 16 kD was detected in the heparin-binding fraction derived from embryos in each stage of blastula, gastrula and 4–8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4–8 somite embryos. In the heparin-binding fraction derived from 12–16 somite embryos neither immunoreactive FGF-1 nor-2 member band was detectable.


fish FGF-1 fish FGF-2 fish embryos cell proliferation heparin affinity Western blot 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References cited

  1. Amaya, E., Stein, P.A., Musci, T.J. and Kirshner, M.W. 1993. FGF signalling in the early specification of mesoderm inXenopus. Development 118: 477–487.Google Scholar
  2. Bradford, C.S., Le Sun and Barnes, D.W. 1994. Basic fibroblast growth factor stimulates proliferation and suppresses melanogenesis in cell cultures derived from early zebrafish embryos. Mol. Mar. Biol. Biotechnol. 3: 78–86.Google Scholar
  3. Burgess, W.H. and Maciag, T. 1989. The heparin-binding (fibroblast) growth factor family of proteins. Ann. Rev. Biochem. 58: 575–606.Google Scholar
  4. Emori, Y., Yasuoka, A. and Saigo, K. 1992. Identification of four FGF receptor genes in medaka fish (Oryzias latipes). FEBS Lett. 314: 176–178.Google Scholar
  5. Esch, F., Bairs, A., Ling, N., Ueno, N., Hill, F., Denoroy, L., Klepper, R., Gospodarowicz, D., Bohlen, P. and Guillemin, R. 1985. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of brain acidic FGF. Proc. Nat. Acad. Sci. U.S.A. 82: 6507–6511.Google Scholar
  6. Fox, G.M., Schiffer, S.G., Rohde, M.F., Tsai, L.B., Banks, A.R. and Arakawa, T. 1988. Production, biological activity, and structure of recombinant basic fibroblast growth factor and an analog with cysteine replaced by serine. J. Biol. Chem. 263: 18452–18458.Google Scholar
  7. Gospodarowicz, D., Neufeld, G. and Schweigerer, L. 1987a. Fibroblast growth factor: structural and biological properties. J. Cell Physiol. Suppl. 5: 15–26.Google Scholar
  8. Gospodarowicz, D., Ferrara, N., Schweigerer, L. and Neufeld, G. 1987b. Structural characterization and biological function of fibroblast growth factor. Endocr. Rev. 8: 95–114.Google Scholar
  9. Hébert, J.M., Basilico, C., Goldfarb, M., Haub, O. and Martin, G.R. 1990. Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev. Biol. 138: 454–463.Google Scholar
  10. Ichimori, Y., Kinosita, Y., Watanabe, T., Seno, M. and Igarashi, K. 1991. Establishment of monoclonal antibodies against human acidic fibroblast growth factor. Biochem. Biophys. Res. Commun. 175: 291–297.Google Scholar
  11. Inaba, K., Seno, M. and Urai, T. 1995. Characterization of heparin-binding mitogenic proteins derived from loach fish embryos. Hyogo Univ. Teachers Education J. (III) 15: 17–22.Google Scholar
  12. Isaacs, H.V., Tannahill, D. and Slack, J.M.W. 1992. Expression of a novel FGF in theXenopus embryo. A new candidate inducting factor for mesoderm formation and anterior specification. Development 114: 711–720.Google Scholar
  13. Iwane, M., Kurokawa, T., Sasada, R., Seno, M., Nakagawa, S. and Igarashi, K. 1987. Expression of cDNA encoding human basic fibroblast growth factor inE. coli. Biochem. Biophys. Res. Commun. 146: 470–477.Google Scholar
  14. Kiefer, P., Mathieu, M., Close, M.J., Peter, G. and Dickson, C. 1993. FGF 3 fromXenopus laevis. EMBO J. 12: 4159–4168.Google Scholar
  15. Kimelman, D. and Kirschner, M. 1987. Synergistic induction of mesoderm by FGF and TGF-β and the identification of an mRNA coding for FGF in the earlyXenopus embryo. Cell 51: 869–877.Google Scholar
  16. Kimelman, D., Abraham, J.A., Haaparanta, T., Palisi, T.M. and Kirschner, M.W. 1988. The presence of fibroblast growth factor in the frog egg: Its role as a natural mesoderm inducer. Science 242: 1053–1056.Google Scholar
  17. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, Lond. 227: 680–685.Google Scholar
  18. Lagente, O., Diry, M. and Courtois, Y. 1986. Isolation of heparin-binding growth factors from dogfish (Mustela canis) brain and retina. FEBS Lett. 202: 207–210.Google Scholar
  19. Prats, H., Kaghad, M., Prats, A.C., Klagsbrun, M., Lélias, J.M., Liauzun, P., Chalon, P., Tauber, J.P., Amarlic, F., Smith, J.A. and Caput, D. 1989. High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons. Proc. Nat. Acad. Sci. U.S.A. 86: 1836–1840.Google Scholar
  20. Seno, M., Iwane, M., Sasada, R., Moriya, N., Kurokawa, T. and Igarashi, K. 1989. Monoclonal antibodies against human basic fibroblast growth factor. Hybrydoma 8: 209–221.Google Scholar
  21. Slack, J.M.W., Darlington, B.G., Heath, J.K. and Gadsave, S.F. 1987. Mesoderm induction in earlyXenopus embryos by heparin-binding growth factor. Nature 326: 197–200.Google Scholar
  22. Suzuki, T., Kurokawa, T. and Asashima, M. 1994. Identification of a heparin-binding, mesoderm-inducing peptide in the swim-bladder of the red seabream,Pagrus major: a probable fish fibroblast growth factor. Fish Physiol. Biochem. 13: 343–352.Google Scholar
  23. Thomas, K., Rios-Candelore, M. and Fitzpatrick, S. 1984. Purification and characterization of acidic fibroblast growth factor from bovine brain. Proc. Nat. Acad. Sci. U.S.A. 81: 357–361.Google Scholar
  24. Watanabe, T., Seno, M., Sasada, R. and Igarashi, K. 1990. Molecular characterization of recombinant human acidic fibroblast growth factor produced inE. coli: comparative studies with human basic fibroblast growth factor. Mol. Endocrinol. 4: 869–879.Google Scholar

Copyright information

© Kugler Publication bv Amsterdam 1996

Authors and Affiliations

  • Kozo Inaba
    • 1
  • Masaharu Seno
    • 2
  • Hisaya Morino
    • 1
  1. 1.Biological InstituteHyogo University of Teachers EducationHyogoJapan
  2. 2.Department of Bioengineering Science, Faculty of EngineeringOkayama UniversityTsushima, OkayamaJapan

Personalised recommendations