Separation of valtrate and isovaltrate by means of preparative liquid chromatography
- 45 Downloads
In this study valtrate and isovaltrate, two isomeric valepotriates present in variousValeriana species, were separated on a preparative scale with a Waters preparative Liquid Chromatography/500 a system. A rapid separation was achieved with the solvent methylene chloriden-propanol-acetone (99 + 0.5 + 0.5). Extensive use of the recycle mode of the instrument resulted in a considerable reduction of the amount of solvent used, without affecting the purity and the recovery (> 90%) of the separated components. With the method presented in this communication 1 gram of a mixture of valtrate and isovaltrate was separated in 20 minutes using 2.1 liters of solvent.
KeywordsPublic Health Methylene Internal Medicine Liquid Chromatography Considerable Reduction
Unable to display preview. Download preview PDF.
- Frosch, F., J. Connert andK. Hilzinger (1978)Deut. Apotheker-Ztg. 118, 1237–1241.Google Scholar
- Hazelhoff, B., B. Weert, R. Denee andTH.M. Malingré (1979)Pharm. Weekbl. Sci. Ed. 1, 140–148.Google Scholar
- Hazelhoff, B., TH.M.Malingré and D.K.F.Meijer.Arch. Intern. Pharmacodyn., submitted for publication.Google Scholar
- Meer, J.H. van, andR.P. Labadie (1980)Planta Medica 39, 269–270.Google Scholar
- Thies, P.W. (168)Tetrahedron 24, 313–347.Google Scholar