Marine Biology

, Volume 99, Issue 3, pp 393–396 | Cite as

Biodepressive effect of zinc on humoral effector of theHolothuria polii immune response

  • C. Canicattí
  • M. Grasso
Article

Abstract

High zinc ion concentrations produce an inhibitory effect on the naturally occurring hemolytic activity of theHolothuria polii coelomic fluid and coelomocyte lysate. The naturally occurring hemagglutinating activity remains unaffected. The inhibition appears reversible, suggesting a non-covalent interaction of the ions with the hemolysins. Moreover, as demonstrated by SDS-PAGE and immunoblotting analysis, the Zn++ treatment does not produce molecular weight variations in the coelomic fluid and coelomocyte lysate hemolytic patterns. This study was conducted during 1987 on samples gathered from the Gulf of Palermo, Italy.

Keywords

Zinc Molecular Weight Immune Response Immunoblotting Analysis Hemolytic Activity 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • C. Canicattí
    • 1
  • M. Grasso
    • 1
  1. 1.Department of BiologyUniversity of LecceLecceItaly

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