Abstract
BALB/c mice were immunised with water extracts made from anEscherichia coli K-12 strain harbouring the shigella invasion plasmid, and hybridomas secreting antibodies specific to invasion plasmid-coded antigens were selected. On Western blots, antibodies produced by one of these clones (MAIC-1) recognised a protein of 43 kDa, which is the molecular mass of invasion plasmid coded antigen C (IpaC). When used in enzyme immunoassay against whole bacterial cells or against proteins secreted by actively growing bacteria, MAIC-1 clearly differentiated between invasive and non-invasive strains. Testing 123 enteroinvasive and 139 non-enteroinvasive strains the MAIC-1-based assay proved to be highly specific and sensitive in recognising enteroinvasive isolates. This test could be an inexpensive and rapid alternative to cumbersome virulence assays and a helpful technique in identifyingShigella or enteroinvasiveEscherichia coli isolates.
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Committee on Issues and Priorities for New Vaccine Development: The burden of disease resulting from diarrhoea. In: Katz SL (ed): New vaccine development: establishing priorities. Volume 2. Diseases of importance in developing countries. National Academy Press, Washington DC, 1986, p. 165.
Ewing WH, Lindberg AA: Serology ofShigella. In: Bergan T (ed): Methods in microbiology, Volume 14. Academic Press, London, 1984, p. 113–142.
Farmer JJ, III, Kelly MT:Enterobacteriaceae. In: Balows A, Hausler WJ, Jr., Hermann KL, Isenberg HD, Shadomy HJ (ed): Manual of clinical microbiology, American Society for Microbiology, Washington DC, 1991, p. 360–383.
Hale TL: Genetic basis of virulence inShigella species. Microbiological Reviews 1991, 55: 206–224.
Brooks JB, Basta MT, El Kholy AM: Studies of metabolites in diarrheal stool specimens containingShigella species by frequency-pulsed electron capture gas-liquid chromatography. Journal of Clinical Microbiology 1985, 21: 599–606.
Kocka FE, Dorigan FD, Abbasi QT, Swiatlo AL, Hubbard-Shepard M: Clinical evaluation of the Wellcolex colourShigella andSalmonella tests. Diagnostic Microbiology and Infectious Diseases 1992, 15: 1–4.
Boileau CR, d'Hauteville HM, Sansonetti PJ: DNA hybridization technique to detectShigella species and enteroinvasiveEscherichia coli. Journal of Clinical Microbiology 1984, 20: 959–961.
Sethabutr O, Echeverria P, Hanchalay S, Taylor DN, Leksomboon U: A non-radioactive DNA probe to identifyShigella and enteroinvasiveEscherichia coli in stools of children with diarrhoea. Lancet 1985, ii: 1095–1097.
Venkatesan MM, Buyssee JM, Kopecko DJ: Use ofShigella flexneri ipaC andipaH gene sequences for the general identification ofShigella spp. and enteroinvasiveEscherichia coli. Journal of Clinical Microbiology 1989, 27: 2687–2691.
Frankel G, Riley L, Giron JA, Valmassoi J, Friedmann A, Strockbine N, Falkow S, Schoolnik GK: Detection ofShigella in feces using DNA amplification. Journal of Infectious Diseases 1990, 161: 1252–1256.
Lampel KA, Jagow JA, Trucksess M, Hill WE: Polymerase chain reaction for detection of invasiveShigella flexneri in food. Applied and Environmental Microbiology 1990, 56: 1536–1540.
Silva RM, Toledo MRF, Trabulsi LR: Biochemical and cultural characteristics of invasiveEscherichia coli. Journal of Clinical Microbiology 1980, 11: 441–444.
Taylor DN, Echeverria S, Pál T, Sethabutr O, Saiborisuth S, Sricharmom S, Rowe B, Cross J: The role ofShigella spp., enteroinvasiveEscherichia coli, and other enteropathogens as causes of childhood dysentery in Thailand. Journal of Infectious Diseases, 1986, 153: 1132–1138.
Serény B: ExperimentalShigella keratoconjunctivitis. Acta Microbiologica Academiae Scientiarum Hungaricae 1955, 2: 293–296.
Hale TL, Bonventre PF:Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infection and Immunity 1979, 24: 879–886.
Pál T, Pácsa S, Emödy L, Vörös S: Antigenic relationship among virulent enteroinvasiveEscherichia coli, Shigella flexneri andShigella sonnei as detected by ELISA. Lancet 1983, ii: 10.
Pál T, Emödy L, Pácsa AS: ELISA detectable virulence marker antigen of enteroinvasiveEscherichia coli is coded by a 140 megadalton plasmid. Acta Microbiologica Hungarica 1986, 33: 341–344.
Pál T, Formal SB, Hale TL: Characterization of virulence marker antigen ofShigella spp. and enteroinvasiveEscherichia coli. Journal of Clinical Microbiology 1989, 27: 561–563.
Li A, Pál T, Forsum U, Lindberg AA: Safety and immunogenicity of the live oral auxotrophicShigella flexneri SFL124 in volunteers. Vaccine 1992, 10: 395–404.
Lindberg AA, Kärnell A, Stocker BAD, Katakura S, Sweiha H, Reinholt FP: Development of an auxotrophic oral liveShigella flexneri vaccine. Vaccine 1988, 6: 146–150.
Oaks EV, Hale TL, Formal SB: Serum immune response toShigella protein antigens inRhesus monkeys and humans infected withShigella spp. Infection and Immunity 1986, 53: 57–63.
Lind SM, Carlin NIA, Lindberg AA: Production and characterization of KDO-specific monoclonal antibodies recognizing lipopolysaccharides from heptoseless mutants ofSalmonella. FEMS Microbiology Letters 1985, 28: 45–49.
Carlin NIA, Lindberg AA: Monoclonal antibodies specific for O-antigenic polysaccharides ofShigella flexneri: clones binding to II, II:3,4, and 7,8 epitopes. Journal of Clinical Microbiology 1983, 18: 1183–1189.
Pál T, Pácsa AS, Emödy L, Vörös S, Sélley E: Modified enzyme-immunosorbent assay for detecting enteroinvasiveEscherichia coli and virulentShigella strains. Journal of Clinical Microbiology 1985, 21: 415–418.
Andrews GP, Hromockyj AE, Coker C, Maurelli AT: Two novel virulence loci,mxiA andmxiB, inShigella flexneri 2a facilitate excretion of invasion plasmid antigens. Infection and Immunity 1991, 59: 1997–2005.
Pál T, Hale TL: Plasmid-associated adherence of Shigellaflexneri in an HeLa cell model. Infection and Immunity 1989, 57: 2580–2582.
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Floderus, E., Pál, T., Karlsson, K. et al. Identification ofShigella and enteroinvasiveEscherichia coli strains by a virulence-specific, monoclonal antibody-based enzyme immunoassay. Eur. J. Clin. Microbiol. Infect. Dis. 14, 111–117 (1995). https://doi.org/10.1007/BF02111868
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DOI: https://doi.org/10.1007/BF02111868