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, Volume 8, Issue 5, pp 488–490 | Cite as

Regional levels of histamine in rat brain after microwave irradiation: Evidence for artifacts in the enzymatic-isotopic assay

  • N. Subramanian
  • W. Schinzel
  • P. Mitznegg
  • C. -J. Estler
Histamine and Kinins

Abstract

Histamine levels in the brain, measured fluorimetrically, were similar for rats killed by decapitation and microwave irradiation. However, enzymatic-isotopic assay yields 5–20 times higher value for microwave irradiation versus decapitation. Thin layer chromatography of the methylated products in the enzymatic-isotopic assay indicated the formation of artifacts following microwave irradiation. The results strongly suggest that a combination of microwave irradiation and enzymatic-isotopic assay is not suitable for histamine analysis in the rat brain.

Keywords

Methylated Histamine Thin Layer Thin Layer Chromatography Microwave Irradiation 

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Copyright information

© Birkhäuser-Verlag 1978

Authors and Affiliations

  • N. Subramanian
    • 1
  • W. Schinzel
    • 1
  • P. Mitznegg
    • 1
  • C. -J. Estler
    • 1
  1. 1.Institut für Pharmakologie und Toxikologie der Universität Erlangen-NürnbergErlangenFederal Republic of Germany

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