Archives of gynecology

, Volume 233, Issue 1, pp 53–61 | Cite as

Immunoperoxidase demonstration of human papilloma virus (HPV) in dysplastic lesions of the uterine cervix

  • K. J. Syrjänen
  • S. Pyrhönen
Original Papers


Routinely processed sections of 47 unselected cervical dysplasias and in situ carcinomas (both condylomatous, CO, and noncondylomatous, NCO) were subjected to staining with indirect immunoperoxidase-PAP technique using anti-human papilloma virus (anti-HPV) immune serum to demonstrate HPV antigens in these lesions. Anti-HPV was raised in guinea pigs immunized with highly purified virions from a pool of human skin wart tissues. Positive reactivity for HPV (brown intranuclear precipitates) was disclosed in 72% of the CO dysplasias and in only one case (5.6%) of the NCO group. The frequency of HPV-positive lesions was inversely related to the degree of epithelial dysplasia, being consistent with the disappearance of ultrastructurally detectable virus particles in lesions turned malignant. HPV-containing cells were characteristic koilocytes or dyskeratotic superficial cells. The present technique provides definite evidence for the HPV etiology of the flat, inverted, and papillomatous condylomas, and seems to offer an advantage as a diagnostic tool of these lesions. When applied to malignant tumors in the near future, the method will undoubtedly bring more light on the suggested role of HPV in the etiology of human squamous cell carcinomas of the uterine cervix and other sites as well.

Key words

Cervical dysplasias Condylomas Anti-HPV immune serum Indirect immunoperoxidase technique Human squamous cell carcinogenesis 


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  1. 1.
    Almeida JD, Oriel JD, Stannard LM (1969) Characterization of the virus found in human genital warts. Microbios 3: 225Google Scholar
  2. 2.
    Boon ME, Fox CH (1981) Simultaneous condyloma acuminatum and dysplasia of the uterine cervix. Acta Cytol 25: 393PubMedGoogle Scholar
  3. 3.
    Casas-Cordero M, Morin C, Roy M, Fortier M, Meisels A (1981) Origin of the koilocyte in condylomata of the human cervix. Ultrastructural study. Acta Cytol 25: 383PubMedGoogle Scholar
  4. 4.
    Della Torre G, Pilotti S, de Palo G, Rilke F (1978) Viral particles in cervical condylomatous lesions. Tumori 64: 549PubMedGoogle Scholar
  5. 5.
    Dunn AEG, Ogilvie MM (1968) Intranuclear virus particles in human genital wart tissue: observations on the ultrastructure of the epidermal layer. J Ultrastruct Res 22: 282PubMedGoogle Scholar
  6. 6.
    Eisinger M, Kucarova O, Sarkar NH, Good RA (1975) Propagation of human wart virus in tissue culture. Nature 256: 432PubMedGoogle Scholar
  7. 7.
    Gissmann L, de Villiers EM, zur Hausen H (1982) Analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 DNA. Int J Cancer 29: 143PubMedGoogle Scholar
  8. 8.
    Grussendorf EI, Bär T (1977) Condyloma acuminata associated with Morbus Bowen (carcinoma in situ). A light- and electron-microscopic study. Dermatologica 155: 50PubMedGoogle Scholar
  9. 9.
    Herrera I, Vallenciano L, Sanchez-Garrido F, Botella-Llusia J (1974) On findings of virus-like structures in uterine cervical carcinoma. Acta Cytol 18: 54Google Scholar
  10. 10.
    Hills E, Laverty CR (1979) Electron-microscopic detection of papilloma virus particles in selected koilocytotic cells in a routine cervical smear. Acta Cytol 23: 53PubMedGoogle Scholar
  11. 11.
    Josey WE, Nahmias AJ, Naib ZM (1976) Viruses and cancer of the lower genital tract. Cancer 38: 526PubMedGoogle Scholar
  12. 12.
    Kurman RJ, Shah KH, Lancaster WD, Jenson AB (1981) Immunoperoxidase localization of papillomavirus antigens in cervical dysplasia and vulvar condylomas. Am J Obstet Gynecol 140: 931PubMedGoogle Scholar
  13. 13.
    Laverty CR, Russell MB, Hills E, Booth N (1978) The significance of noncondylomatous wart virus infection of the cervical transformation zone. A review with discussion of two illustrative cases. Acta Cytol 22: 295Google Scholar
  14. 14.
    Ludwig ME, Lowell DM, Livolsi VA (1981) Cervical condylomatous atypia and its relationship to cervical neoplasia. Am J Clin Pathol 76: 255PubMedGoogle Scholar
  15. 15.
    Meisels A, Roy M, Fortier M, Morin C (1979) Condylomatous lesions of the cervix. Morphologic and colposcopic diagnosis. Am J Diagn Gynecol Obstet 1: 109Google Scholar
  16. 16.
    Meisels A, Roy M, Fortier M, Marin C, Casas-Cordero M, Shah KV, Turgeon H (1981) Human papillomavirus infection of the cervix. The atypical condyloma. Acta Cytol 25: 7PubMedGoogle Scholar
  17. 17.
    Meisels A. Morin C (1981) Human papillomavirus and cancer of the uterine cervix. Gynecol Oncol 12: 111Google Scholar
  18. 18.
    Morin C, Braun L, Casas-Cordero M, Shah KV, Roy M, Fortier M, Meisels A (1981) Confirmation of the papillomavirus etiology of condylomatous cervix lesions by the peroxidase-antiperoxidase techniques. JNCI 66: 831PubMedGoogle Scholar
  19. 19.
    Nasemann T, Schaeg G (1976) Electron microscopic examination of condylomata acuminata. J Cutan Pathol 3: 147Google Scholar
  20. 20.
    Purola E, Savia E (1977) Cytology of gynecologic condyloma acuminatum. Acta Cytol 21: 26PubMedGoogle Scholar
  21. 21.
    Pyrhönen S, Penttinen K (1972) Wart-virus antibodies and the prognosis of wart disease. Lancet 2: 1330PubMedGoogle Scholar
  22. 22.
    Pyrhönen S (1978) Antibody response against human papilloma viruses. MD Thesis, HelsinkiGoogle Scholar
  23. 23.
    Recher L, Srebnik E (1981) Histopathologic features of koilocytic atypia. A detailed description. Acta Cytol 25: 377PubMedGoogle Scholar
  24. 24.
    Ruiter M, van Mullem P (1966) The association of virus particles with malignant changes in the skin in a case of epidermodysplasia verruciformis. J Invest Dermatol 47: 247PubMedGoogle Scholar
  25. 25.
    Shah KH, Lewis MG, Jenson AB, Kurman RJ, Lancaster WD (1980) Papillomavirus and cervical dysplasia. Lancet 2: 1190Google Scholar
  26. 26.
    Syrjänen KJ (1979) Morphologic survey of the condylomatous lesions in dysplastic and neoplastic epithelium of the uterine cervix. Arch Gynecol 227: 153PubMedGoogle Scholar
  27. 27.
    Syrjänen KJ (1979) Histological and cytological evidence of a condylomatous lesion associated with an invasive carcinoma of uterine cervix. Arch Geschwulstforsch 49: 436PubMedGoogle Scholar
  28. 28.
    Syrjänen KJ (1980) Condylomatous lesions in dysplastic and neoplastic epithelium of the uterine cervix. Surg Gynecol Obstet 150: 372PubMedGoogle Scholar
  29. 29.
    Syrjänen KJ (1980) Condylomatous epithelial changes in uterine cervix and their relationship to cervical carcinogenesis. Int J Gynaecol Obstet 17: 415PubMedGoogle Scholar
  30. 30.
    Syrjänen KJ (1980) Current views on the condylomatous lesions in uterine cervix and their possible relationship to cervical squamous cell carcinoma. Obstet Gynecol Surv 35: 685PubMedGoogle Scholar
  31. 31.
    Syrjänen KJ (1980) Condylomatous lesions of the uterine cervix with special reference to squamous cell carcinogenesis. Gynecol Obstet Invest 11: 350PubMedGoogle Scholar
  32. 32.
    Syrjänen KJ, Heinonen UM, Kauraniemi T (1981) Cytological evidence of the association of condylomatous lesions with the dysplastic and neoplastic changes in uterine cervix. Acta Cytol 25: 17PubMedGoogle Scholar
  33. 33.
    Syrjänen KJ, Syrjänen SM (1981) Premalignant epithelial changes in the uterine cervix of women aged less than thirty with special emphasis on the coexistent condylomatous lesions. IJDV&L 47: 88Google Scholar
  34. 34.
    Syrjänen KJ, Pyrhönen S (1982) Demonstration of human papilloma virus antigen in the condylomatous lesions of the uterine cervix by immunoperoxidase technique. Gynecol Obstet Invest 14: 90PubMedGoogle Scholar
  35. 35.
    Woodruff JD, Braun L, Cavalieri R, Gupta P, Pass F, Shah KV (1980) Immunologic identification of papillomavirus antigen in condyloma tissues from the female genital tract. Obstet Gynecol 56: 727PubMedGoogle Scholar
  36. 36.
    Yabe Y, Koyama H (1973) Virus and carcinogenesis in epidermodysplasia verruciformis. Gann 64: 167PubMedGoogle Scholar
  37. 37.
    zur Hausen H (1977) Human papillomaviruses and their possible role in squamous cell carcinomas. Curr Top Microbiol Immunol 78: 1PubMedGoogle Scholar

Copyright information

© J. F. Bergmann Verlag 1982

Authors and Affiliations

  • K. J. Syrjänen
    • 1
  • S. Pyrhönen
    • 2
  1. 1.Dept. of PathologyUniversity of KuopioKuopioFinland
  2. 2.Dept. of VirologyUniversity of HelsinkiHelsinkiFinland

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