Journal of Bioenergetics and Biomembranes

, Volume 28, Issue 2, pp 191–198 | Cite as

The high-conductance channels of yeast mitochondrial outer membranes: A planar bilayer study

  • György Bàthori
  • IldikÒ SzabÒ
  • Daniel Wolff
  • Mario Zoratti
Research Articles

Abstract

The high-conductance channels present in the outer membranes of wild-type and porin-less yeast mitochondria have been characterized electrophysiologically after incorporation in planar bilayer membranes. The most prominent activity was ascribed to a voltage-dependent, substaterich, cationic channel which generally inactivated at potentials positive in thecis compartment, in agreement with the observations from patch-clamp experiments on porin-less mitoplasts. This channel has been identified as the so-called “peptide-sensitive channel” (PSC). We also observed similar channels displaying either no inactivation, or inactivation at both positive and negative potentials. These latter properties match those already described for mammalian and yeast PSC, respectively. These different behaviors are tentatively explained as arising from the presence, or lack of, peptides bound to the PSC. Very high conductances, apparently due to cooperative gating, were frequently displayed. In wild-type membranes, activity ascribable to the porin was also observed.

Key words

Electrophysiology peptide-sensitive channel mitochondrial megachannel porin planar bilayer yeast mitochondria 

Abbreviations used

PSC

peptide-sensitive channel

MMC

mitochondrial megachannel

VDAC

voltage-dependent anion channel (mitochondrial porin)

Hepes

4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid

pCOxIV

pre-cytochrome oxidase subunit IV

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • György Bàthori
    • 1
  • IldikÒ SzabÒ
    • 1
  • Daniel Wolff
    • 1
  • Mario Zoratti
    • 1
  1. 1.Dip. Scienze Biomediche SperimentaliCentro CNR per le BiomembranePadovaItaly

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