Journal of Oceanography

, Volume 42, Issue 6, pp 481–486 | Cite as

Growth inhibition of a red tide flagellate,Chattonella antiqua by copper

  • Yasuo Nakamura
  • Kazuhiro Sawai
  • Masataka Watanabe


Copper toxicity inChattonella antiqua (Raphidophyceae) was examined using an artificial seawater medium. The growth rate (Μ) was found to be a unique function of cupric ion activity (acu) as follows:
$$\mu = \mu _{\max } /(1 + K(a_{Cu} )^2 ),$$
whereΜmax andK are. 0.63 d−1 and 2.4×1020mol−2l2, respectively. The value ofacu at whichΜ is reduced to half the maximum is 10−10.2 M.

A comparison of our results with those for other phytoplankton species indicatedC. antiqua to be rather sensitive to cupric ions. Furthermore, the growth ofC. antiqua was strongly influenced at the calculated cupric ion activity of natural seawater in the Seto Inland Sea, assuming only inorganic copper complexation. Thus, organic chelation may be necessary beforeC. antiqua is capable of competing with other phytoplankton species.


Phytoplankton Phytoplankton Species Natural Seawater Graphite Furnace Atomic Absorption Spectrophotometry Artificial Seawater Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, D.M. and F.M.M. Morel (1978): Copper sensitivity ofGonyaulax tamarensis. Limnol. Oceanogr.,23, 283–295.CrossRefGoogle Scholar
  2. Anderson, D.M., J.S. Lively and R.F. Vaccaro (1984): Copper complexation during spring blooms in coastal waters. J. Mar. Res.,42, 677–695.CrossRefGoogle Scholar
  3. Fisher, N.S., G.J. Jones and D.M. Nelson (1981): Effects of copper and zinc on growth, morphology, and metabolism ofAsterionella japonica (Cleve). J. Exp. Mar. Biol. Ecol.,51, 37–56.CrossRefGoogle Scholar
  4. Gavis, J. (1983): Toxic binding of cupric ion by marine phytoplankton. J. Mar. Res.,41, 53–63.CrossRefGoogle Scholar
  5. Gavis, J., R.R.L. Guillard and B.L. Woodward (1981): Cupric ion activity and the growth of phytoplankton clones isolated from different marine environments. J. Mar. Res.,39, 315–333.Google Scholar
  6. Guillard, R.R.L. and J.H. Ryther (1962): Studies of marine planktonic diatoms. I.Cyclotella nana Hustedt andDetonula confervacea (Cleve) Gram. Can. J. Microbiol.,8, 229–239.CrossRefGoogle Scholar
  7. Huntsman, S.A. and W.G. Sunda (1980): The role of trace metals in regulating phytoplankton growth with emphasis on Fe, Mn and Cu. p. 285–328. In: Physiological Ecology of Phytoplankton, ed. by I. Morris, Blackwell Scientific Publications.Google Scholar
  8. Nakamura, Y. (1985a): Ammonium uptake kinetics and interactions between nitrate and ammonium uptake inChattonella antiqua. J. Oceanogr. Soc. Japan,41, 33–38.CrossRefGoogle Scholar
  9. Nakamura, Y. (1985b): Kinetics of nitrogen- or phosphorus-limited growth and effects of growth conditions on nutrient uptake inChattonella antiqua. J. Oceanogr. Soc. Japan,41, 381–387.CrossRefGoogle Scholar
  10. Nakamura, Y. and M.M. Watanabe (1983a): Growth characteristics ofChattonella antiqua (Raphidophyceae) Part 1. Effects of temperature, salinity, light intensity and pH on growth. J. Oceanogr. Soc. Japan,39, 110–114.CrossRefGoogle Scholar
  11. Nakamura, Y. and M.M. Watanabe (1983b): Nitrate and phosphate uptake kinetics ofChattonella antiqua grown in light/dark cycles. J. Oceanogr. Soc. Japan,39, 167–170.CrossRefGoogle Scholar
  12. Ono, C. and H. Takano (1980):Chattonella antiqua (Hada) comb. nov., and its occurrence on the Japanese coast. Bull. Tokai Reg. Fish. Res. Lab.,102, 93–100.Google Scholar
  13. Segar, D.A. and A.Y. Cantillo (1975): Direct determination of trace metals in seawater by flameless atomic absorption spectrophotometry. p. 56–81. In: Analytical Methods in Oceanography. Advances in Chemistry Series,147, ed. by T.R.P. Gibb, Jr., American Chemical Society.Google Scholar
  14. Stumm, W. and J.J. Morgan (1970): Acids and bases. p. 69–117. In: Aquatic Chemistry, John Wiley & Sons.Google Scholar
  15. Sunda, W.G. and P.A. Gillespie (1979): The response of a marine bacterium to cupric ion and its use to estimate cupric ion activity. J. Mar. Res.,37, 761–777.Google Scholar
  16. Sunda, W.G. and R. R. L. Guillard (1976): The relationship between cupric ion activity and the toxicity of copper to phytoplankton. J. Mar. Res.,34, 511–529.Google Scholar
  17. Sunda, W.G. and J.A.M. Lewis (1978): Effects of complexation by natural organic ligands on the toxicity of copper to a unicellular alga,Mono-chrysis lutheri. Limnol. Oceanogr.,23, 870–876.CrossRefGoogle Scholar
  18. Sunda, W.G., R.T. Barber and S.A. Huntsman (1981): Phytoplankton growth in nutrient rich seawater: importance of copper-manganese callular interactions. J. Mar. Res.,39, 567–586.Google Scholar
  19. Westall, J.C., J.K. Zachary and F.M.M. Morel (1976): MINEQL, a computer program for the celculation of chemical equilibrium composition of aqueous systems. Technical Note. No. 8, Water Quality Lab., Ralph M. Parson Laboratory for Water Resources and Hydrodynamics. Dept. of Civil Engineering, M.I.T., Cambridge, MA.Google Scholar

Copyright information

© Oceanographical Society of Japan 1986

Authors and Affiliations

  • Yasuo Nakamura
    • 1
  • Kazuhiro Sawai
    • 1
  • Masataka Watanabe
    • 2
  1. 1.Laboratory of Marine EnvironmentThe National Institute for Environmental StudiesIbarakiJapan
  2. 2.Faculty of EngineeringScience University of TokyoTokyoJapan

Personalised recommendations