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Patch-clamp study of vascular plant chloroplasts: Ion channels and photocurrents

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Abstract

This article presents direct measurements of large-conductance cation channel (G=730 pS in 100/50mM KCl;\(P_{K^ + } /P_{Cl^ - } \) =2.8) and light-induced current (photocurrent) in chloroplasts from C4 plants,Amaranthus hybridus andZea mays, using the conventional patch-clamp technique. It was shown that preillumination of chloroplast gave rise to a fast decaying transient (τ ≅ 6 ms) in the light-induced current for the second and following light pulses. This transient increase of photocurrent was interpreted as a consequence of photoreducing of the redox pool between photosystems.

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Muñiz, J.J., Pottosin, I.I. & Sandoval, L. Patch-clamp study of vascular plant chloroplasts: Ion channels and photocurrents. J Bioenerg Biomembr 27, 249–258 (1995). https://doi.org/10.1007/BF02110040

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