Acetylene reduction in the rhizosphere of rice: Methods of assay
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A method is described for thein situ assay of acetylene reduction activity in the rhizosphere of rice involving the use of inexpensive, lightweight equipment. The assay is quick to set up and thus many replicates can be run simultaneously. Control of any acetylene-reducing blue-green algae in the assay chamber is achieved with a photosynthetically active herbicide which has no measurable effect on rhizosphere activity. Results using this method are compared with those from an excised root assay of the same plants. Only a very weak correlation between the two methods was found.
KeywordsPlant Physiology Acetylene Weak Correlation Measurable Effect Reduction Activity
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- 1.Balandreau, J. P. and Dommergues, Y. R., Microbiologie du sol — Mesurein situ de l'activité nitrogenasique. C.R. Acad. Sci. (Paris), ser. D273, 2020–2023 (1971).Google Scholar
- 2.Balandreau, J. P. and Dommergues, Y. R., Assaying nitrogenase (C2H2) activity in the field. Bull. Ecol. Res. Comm. (Stockholm)17, 247–254 (1973).Google Scholar
- 4.Balandreau, J. P., Rinaudo, G., Oumarov, M. M. and Dommergues, Y. R., Nitrogen fixation in paddy soils.In Nitrogen Fixation by Free-living Microorganisms. (W. D. P. Stewart Ed.) Cambridge University Press, p. 611–628 (1975).Google Scholar
- 5.Brown, C. B. and Bally, G. S., Soils of central Trinidad. Land capability survey of Trinidad and Tobago, No 4. Gov't Printery, Trinidad and Tobago (1970).Google Scholar
- 6.Burris, R. H., Methodology.In The Biology of Nitrogen Fixation. (A. Quispel Ed.) New Holland, Amsterdam, p. 9–36 (1974).Google Scholar
- 10.IRRI, Annual report of the microbiology section of the International Rice Research Institute, Los Banos, Laguna, Phillipines (1976).Google Scholar
- 11.Lee, K.-K. and Yoshida, T., An assay technique of measurement of nitrogenase activity in root zone of rice for varietal screening by the acetylene reduction method. Plant and Soil46, 127–134 (1977).Google Scholar
- 12.Smith, R. J. Jr., Responses of rice to postemergence treatments of Propanil. Weed Sci.22, 563–568 (1974).Google Scholar
- 13.Watanabe, I. and Lee, K-K., Non-symbiotic nitrogen fixation in rice and rice paddies. Paper presented at ‘Biological nitrogen fixation in farming systems of the humid tropics’ Symp. 20th–23rd Oct. 1975, at I.I.T.A., Ibadan. Nigeria (1975).Google Scholar
- 14.Yoshida, T. and Ancajas, R. R., Nitrogen fixation by bacteria in the root zone of rice. Soil Sci. Soc. Am. Proc.35, 156–158 (1971).Google Scholar
- 15.Yoshida, T. and Ancajas, R. R., Nitrogen fixing activity in upland and flooded rice fields. Soil Sci. Soc. Am. Proc.37, 42–46 (1973).Google Scholar