Plant and Soil

, Volume 50, Issue 1–3, pp 567–574 | Cite as

Acetylene reduction in the rhizosphere of rice: Methods of assay

  • Robert M. Boddey
  • Peter Quilt
  • Nazeer Ahmad


A method is described for thein situ assay of acetylene reduction activity in the rhizosphere of rice involving the use of inexpensive, lightweight equipment. The assay is quick to set up and thus many replicates can be run simultaneously. Control of any acetylene-reducing blue-green algae in the assay chamber is achieved with a photosynthetically active herbicide which has no measurable effect on rhizosphere activity. Results using this method are compared with those from an excised root assay of the same plants. Only a very weak correlation between the two methods was found.


Plant Physiology Acetylene Weak Correlation Measurable Effect Reduction Activity 
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Copyright information

© Martinus Nijhoff 1978

Authors and Affiliations

  • Robert M. Boddey
    • 1
  • Peter Quilt
    • 1
  • Nazeer Ahmad
    • 1
  1. 1.Department of Soil ScienceUniversity of the West IndiesSt AugustineTrinidad

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