Current Microbiology

, Volume 22, Issue 1, pp 73–76 | Cite as

Regulation of isocitrate lyase inRhodobacter capsulatus E1F1

  • Rafael Blasco
  • Jacobo Cárdenas
  • Francisco Castillo


InRhodobacter capsulatus E1F1, isocitrate lyase (ICL) (EC is a regulatory enzyme whose levels are increased in the presence of acetate as the sole carbon source. Acetate activated isocitrate lyase in a process dependent on energy supply and de novo protein synthesis. In contrast to isocitrate lyase, isocitrate dehydrogenase (ICDH) activity was independent of the carbon source used for growth and significantly increased in darkened cells. Pyruvate or yeast extract prevented in vivo activation of isocitrate lyase in cells growing on acetate. The enzyme was reversibly inactivated to a great extent in vitro by pyruvate and other oxoacids presumably involved in acetate metabolism. These results suggest that, inR. capsulatus E1F1, isocitrate lyase is regulated by both enzyme synthesis and oxoacid inactivation.


Enzyme Acetate Carbon Source Protein Synthesis Pyruvate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Albers H, Gottschalk G (1976) Acetate metabolism inRhodopseudomonas gelatinosa and several otherRhodospirillaceae. Arch Microbiol 111:45–49PubMedGoogle Scholar
  2. 2.
    Blasco R, Cárdenas J, Castillo F (1989) Acetate metabolism in purple non sulfur bacteria. FEMS Microbiol Lett 58:129–132Google Scholar
  3. 3.
    Cooper TG (1977) The Tools of Biochemistry. New York/London/Sidney: J Wiley and Sons, pp 51–53Google Scholar
  4. 4.
    Eley JH, Knobloch K, Han T-W (1979) Effect of growth condition on enzymes of the citric acid cycle and the glyoxylate cycle in the photosynthetic bacteriumRhodopseudomonas palustris. Antonie van Leeuwenhoek J Microbiol 45:521–529PubMedGoogle Scholar
  5. 5.
    Garnak M, Reeves HC (1979) Purification and properties of phosphorylated isocitrate dehydrogenase ofEscherichia coli. J Biol Chem 254:7915–7920PubMedGoogle Scholar
  6. 6.
    Golberg DM, Ellis G (1983) Isocitrate dehydrogenase. In: Bergmeyer HU, Bergmeyer J, Grass M (eds) Methods of enzymatic analysis, vol. III. Weinheim: Verlag-Chemie, pp 183–190Google Scholar
  7. 7.
    Hoyt JC, Reeves HC (1988) In vivo phosphorylation of isocitrate lyase fromEscherichia coli D5H3G7. Biochem Biophys Res Commun 153:875–880PubMedGoogle Scholar
  8. 8.
    Kornberg HL (1966) The role and control of the glyoxylate cycle inEscherichia coli. Biochem J 99:1–11PubMedGoogle Scholar
  9. 9.
    Kornberg HL, Madsen NB (1958) The metabolism of C2 compounds in micro-organisms. 3. Synthesis of malate from acetate via the glyoxylate cycle. Biochem J 68:549–557PubMedGoogle Scholar
  10. 10.
    Lamprecht W, Heinz F (1984) Pyruvate. In: Bergmeyer HU, Bergmeyer J, Grass M (eds) Methods of enzymatic analysis, vol. VI. Weinheim: Verlag-Chemie, pp 570–577Google Scholar
  11. 11.
    Lopez Boado YS, Herrero P, Fernández T, Fernández R, Moreno F (1988) Glucose-stimulated phosphorylation of yeast isocitrate lyasein vivo. J Gen Microbiol 134:2499–2505PubMedGoogle Scholar
  12. 12.
    Nielsen AM, Sojka GA (1979) Photoheterotrophic utilization of acetate by the wild type and an acetate-adapted mutant ofRhodopseudomonas capsulata. Arch Microbiol 120:39–42Google Scholar
  13. 13.
    Nielsen AM, Rampsch BJ, Sojka GA (1979) Regulation of isocitrate lyase in a mutant ofRhodopseudomonas capsulata adapted to growth on acetate. Arch Microbiol 120:43–46Google Scholar
  14. 14.
    Rej R (1985) Oxaloacetate. UV-method. In: Bergmeyer HU, Bergmeyer J, Grass M (eds) Methods of enzymatic analysis, vol. VII. Weinheim: Verlag-Chemie, pp 59–67Google Scholar
  15. 15.
    Robertson EF, Hoyt JC, Reeves HC (1988) Evidence of histidine phosphorylation in isocitrate lyase fromEscherichia coli. J Biol Chem 263:2477–2482PubMedGoogle Scholar
  16. 16.
    Weaver PF, Wall JD, Gest H (1975) Characterization ofRhodopseudomonas capsulata. Arch Microbiol 105:207–216PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1991

Authors and Affiliations

  • Rafael Blasco
    • 1
  • Jacobo Cárdenas
    • 1
  • Francisco Castillo
    • 1
  1. 1.Departmento de Bioquímica y Biología Molecular y Fisiología, Facultad de Ciencias, Avda. San Alberto Magno s/nUniversidad de CórdobaCórdobaSpain

Personalised recommendations